蓖麻毒蛋白A链基因克隆与重复表达载体构建  

Ricin a chain gene cloning in Ricinus commol/Lunis L. and the construction of its repeat expression vectors

作　　者：黄凤兰[1,2] 李国瑞[3] 萨日娜[3] 陈永胜[2] 阎秀峰[1] 

机构地区：[1]东北林业大学生命科学学院,哈尔滨黑龙江150000 [2]内蒙古民族大学生命科学学院,内蒙古通辽028000 [3]内蒙古民族大学农学院,内蒙古通辽028000

出　　处：《中国油料作物学报》2009年第2期167-172,共6页Chinese Journal of Oil Crop Sciences

基　　金：国家自然科学基因项目(30760123)

摘　　要：利用降落PCR的方法,从蓖麻基因组DNA中克隆毒蛋白A链基因的560bp片段;利用一步法将该基因的2个正义片段和2个反义片段分别与pB I-121质粒连接,构建含该基因的正义重复和反义重复表达载体,为利用共抑制技术抑制蓖麻毒蛋白A链基因表达的研究奠定基础。To clone the RTA gene from genome DNA of Ricinus commol/Lunis L. by touch down PCR. The expected 560bp gene fragment was obtained. Two RTA sense fragment and two RTA antisense fragment was linked to the plasmid pBI-121 respectively by one step. The sense and antisense repeat expression vector were constructed. This result provided a foundation for further research of RTA gene expression by co-suppression technology.

关 键 词：蓖麻毒蛋白 重复表达载体 降落PCR 一步法 共抑制 

分 类 号：S565.603[农业科学—作物学]