髓系细胞触发受体1结合多肽与脓毒症炎症反应  

Triggering receptor expressed on myeloid cell-I binding peptide and inflammation of sepsis

作　　者：张彦峰[1] 莫红缨[1] 刘晓青[1] 何为群[1] 黎毅敏[1] 

机构地区：[1]广州医学院第一附属医院广州呼吸疾病研究所,510120

出　　处：《国际呼吸杂志》2009年第13期769-771,F0003,共4页International Journal of Respiration

基　　金：基金项目：广东省自然科技基金项目（061109）

摘　　要：目的研究髓系细胞触发受体1（triggering receptor expressed on myeloid cell-1，TREM-1）结合多肽对脓毒症小鼠保护作用。方法经噬菌体表面展示肽库筛选出的TREM-1结合多肽，42只健康雄性Balb／c小鼠随机分为空白组，脂多糖（LPS）组，对照1（多肽）组，对照2（LPS＋TREM-1单抗）组，对照3（LPS＋多肽＋TREM-1单抗）组，早期治疗（LPS＋LPS前1h多肽）组，延迟治疗（LPS＋LPS后4h多肽）组，各组分别于设定时间点麻醉处死小鼠，取肝组织提mRNA行逆转录、荧光定量PCR检测TREM-1、肿瘤坏死因子α（TNF—α）、白介素1β（IL-1β）mRNA表达水平，并取肺组织病理组织学观察。结果LPS能增加小鼠肝细胞TREM-1mRNA表达，早期治疗组可抑制LPS引起的肝组织TNF—α、IL-1βmRNA增加且能减轻LPS导致的肺病理组织炎症反应而延迟治疗组并未见以上抑制作用，此抑制作用能为TREM-1单抗所拮抗。结论LPS诱导的脓毒症可见TREM-1mRNA表达上调。TREM-1结合多肽对脓毒症小鼠具有保护作用，阻断TREM-1介导的全身炎症反应综合征可能是其发挥作用的机制。Objective To study the influence of triggering receptor expressed on myeloid cell-1 （TREM-1） binding peptide on lipopolysaccharide （LPS）-induced sepsis in mice. Methods Mice sepsis models were established by LPS through peritoneal injection. Mice were divided into blank, LPS-induced sepsis,binding peptide, LPS＋ TREM-1 monoclonal antibody, LPS＋ TREM-1 monoclonal antibody ＋ binding peptide protection,early binding peptide protection and delayed binding peptide protection. Lung and liver were obtained at the set time. The level of liver TREM-1, TNF-α, IL-1β mRNA were determined by quantitative reverse transcriptional polymerase chain reaction. Detection of histology for lung was carried out. Results TREM-1 mRNA increased while the mice were treated with LPS. The early binding peptide protection could decrease the liver TNF-α, IL-1β mRNA, reducing lung histological damage, which was inhibited by TREM-1 monoclonal agonist antibodies and not detected in the delayed protection group. Conclusions Treating the mice with LPS resulted in increased level of TREM-1 mRNA. TREM-1 binding peptide has obviously protective effect on LPS-induced sepsis in mice. The blockage of SIRS induced by TREM-1 may be a new pathway for the treatment of sepsis.

关 键 词：髓系细胞触发受体1 脓毒症 结合多肽 

分 类 号：R45[医药卫生—治疗学]