HPLC法测定酮洛芬在大鼠血浆中的浓度  

Determination of Ketoprofen in rat plasma by HPLC

作　　者：刘静[1] 田大丰[1] 田晓琳[1] 莫凤奎[1] 王中彦[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《现代生物医学进展》2009年第12期2339-2341,2356,共4页Progress in Modern Biomedicine

摘　　要：目的：建立大鼠血浆中酮洛芬的HPLC测定方法，进行酮洛芬微乳凝胶的药物动力学研究。方法：血浆样品采用甲醇沉淀蛋白方法处理，萘普生钠为内标；色谱柱为Diamosil C18（200mm×4．6mmi．d，5μm），流动相为甲醇-0．01mol·L-1磷酸二氢钾（70：30，磷酸调pH3．0），流速1mL·min-1，检测波长为255nm。进样量为20μL，柱温为室温。结果酮洛芬与内标萘普生钠完全分离且血浆中内源，陆物质对酮洛芬的含量测定无影响；酮洛芬在O．20-50μg·mL-1范围内线性关系良好，r=0．9998；最低定量限为0．05μg·mL-1；日内和日间精密度均（RSD）小于2．4％；回收率为91．3％-107．2％。结论：所用方法灵敏、准确、重复性好、回收率高，可用于酮洛芬微乳凝胶的血药浓度检测。Objective： To establish an HPLC method for the determination ofketoprofen in rat plasma, and study the pharmacoki- netic of ketoprofen in rat after transdermal administration. Methods： Protein in plasma sample was deposited by methanol. Ketoprofen and internal standard, naproxen sodium were analyzed on a DiamosilCl8 column （200 min× 4.6 mm i.d, 5 μm） with a mobile phase con- sisted of methanol -0.01 mol·L-1 phosphate buffer salt （70：30, v/v; adjusted to pH=3.0 using phosphoric acid ）. The flow rate was 1.0 mL·min-1; the detection wavelength was at 255 nm. The sample size was 20 μL, and the column temperature was set at room tempera- ture. Results： Ketoprofen and naproxen sodium were separated completely without interference. The assay was linear over the concentra- tion range of 0.20-50 μg· mL-1 in plasma. The intm- and inter- day precisions were below 2.4%. The average relative recoveries were 91.3%-107.2% in plasma. The lower limits of quantification in bio-samples was 0.05 μg·mL-1 Conclusions： The method was successfully applied to determine the ketoprofen concentration in rat plasma after transdermal administration of ketoprofen microemulsion-based gel. The method was accurate, good reproducible and high recovery.

关 键 词：酮洛芬 HPLC 微乳凝胶 血药浓度 

分 类 号：Q95-3[生物学—动物学] R927.2[医药卫生—药学]