高效液相色谱法测定肌肽滴眼液中L-肌肽的含量及有关物质  

Determination of L-carnosine in Carnosine Eye Drops and Its Related Substances by HPLC

作　　者：高珣[1] 陈晓辉[1] 韩木南[1] 毕开顺[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《现代生物医学进展》2009年第12期2345-2346,共2页Progress in Modern Biomedicine

摘　　要：目的：建立肌肽滴眼液中L-肌肽的含量测定的HPLC方法。方法：采用Kromasil NH2色谱柱（200mm×4．6mm，5μm），流动相为乙腈-40mmol·L^-1酸氢二钾溶液（44：56，磷酸调pH6.3），流速1．0mL·min^-1,检测波长为210nm，柱温为35℃。结果：肌肽在9．93-99.3μg·mL^-1范围内线性关系良好（-0．9998），平均回收率为99．8％（RSD=1．0％，n=9），滴眼液中含L-肌肽的标示百分含量为97．0％-102．0％。结论：该方法操作简便、重复性好、专属性强，可用作肌肽滴眼液中L-肌肽的质量控制。Objective： To establish a HPLC method for determination the content of L-camosine and its related substances in carnosine eye drops. Methods： The separation was performed on a Kromasil NH2 column （200 mm × 4.6 mm, 5 μm）. The mobile phase was acetonitrile-40 mmol. L-1 dibasic potassium phosphate, （44：56,adjusted pH to 6.3 with Phosphoric acid）.The flow rate was 1.0 mL- min-t. Ultraviolet absorption detector was set at 210 nm and column temperature at 35 ℃. Results： The linear range of L-camosine was between 9.93 and 99.3μg·mL-1 （r = 0.9998）. The average recovery was 99.8 % with RSD was 1.0 %. The Labeled Content （%） of L-carnosine in carnosine eye drops was between 97.0% and 102.0%. The related substances of L-camosine eye drops can be completely separated from L-camosine. Conclusions： A reproducible and specific method is provided for the quality control of carnosine eye drops.

关 键 词：高效液相色谱法 L-肌肽 含量测定 有关物质 肌肽滴眼液 

分 类 号：R927.2[医药卫生—药学]