裸DNA治疗研究  

Research progress of naked DNA therapy

作　　者：付爱玲[1] 

机构地区：[1]西南大学药学院,重庆400715

出　　处：《生命的化学》2009年第3期327-330,共4页Chemistry of Life

基　　金：西南大学科研基金项目(10429020710903)资助

摘　　要：目前基因治疗最简单安全的方法是直接使用裸DNA。为使裸DNA在体内能持久高效表达,人们通过尝试不同的输送方式、研究质粒DNA骨架结构,以及探索裸DNA进入体细胞的机制等,以期找到这个具有潜在药用价值的物质的有效使用方法。目前裸DNA在细胞摄取机制和开发持久高效的表达载体方面上取得了一些进展,这为以后使用裸DNA临床治疗外周疾病和中枢神经系统疾病奠定了基础。At present, naked DNA represents the simplest and safest vehicle for gene therapy. The clinical usefulness of naked DNA has been hindered by their relatively low gene delivery and transgene expression efficiency. In order for naked DNA to achieve persistent high level of transgene expression, different delivery methods, backbone of naked DNA, and molecular mechanisms of DNA uptake in mammalian cells are currently under investigation. Some progress has been made in the mechanisms of DNA uptake and the development of new vehicles for expression of persistent, high levels of transgene in vivo, which has laid a basis for clinic use of naked DNA in peripheral and central nervous system diseases.

关 键 词：裸DNA 输送方式 细胞摄取机制 

分 类 号：Q789[生物学—分子生物学]