颗粒状甲烷单加氧酶的结构与催化机理  

Structure and catalytic mechanism of particulate methane monooxygenase

作　　者：郑维[1,2] 权春善[1,2] 王军华[1,2] 马金龙[1,2] 范圣第[1,2] 

机构地区：[1]大连民族学院生物技术与资源利用国家民委-教育部重点实验室,大连116600 [2]大连民族学院生命科学学院,大连116600

出　　处：《生命的化学》2009年第3期347-351,共5页Chemistry of Life

基　　金：辽宁省自然科学基金项目(202001);大连民族学院青年基金项目(2007A101)资助

摘　　要：颗粒状甲烷单加氧酶(pMMO)是存在于嗜甲烷菌膜上的一种金属酶,具有将甲烷氧化为甲醇、降解环境污染物等多种功能。由于这种酶难于分离和纯化,阻碍了其在结构和催化机理方面的研究,因而影响了实际应用。本文介绍了近几年颗粒状甲烷单加氧酶的结构和催化机理方面的研究进展,着重阐述其金属中心的结构及催化活性基础。Particulate methane monooxygenase is a membrane-bound enzyme that catalyzes the oxidation of methane to methanol in methanotrophs, pMMO is expressed when metal ion is available, and its active site is believed to contain copper. Whereas sMMO is well characterized, most aspects of pMMO biochemistry remain unknown and somewhat controversial. This review emphasizes the advances in the past ten years in studies related to pMMO and copper-dependent regulation in methanotrophs. The pMMO metal centers have been characterized spectroscopically, and the first pMMO crystal structure has been determined.

关 键 词：颗粒状甲烷单加氧酶 三聚体结构 金属中心 催化机理 

分 类 号：Q936[生物学—微生物学]