Caveolin-3在心房纤颤组织中的表达  

Expression of Caveolin-3 in atrial tissues with atrial fibrillation

作　　者：陈艳艳[1] 张莹[1] 王宁[1] 路慧敏[1] 梁海海[1] 王然[1] 沐晓芹[1] 吕延杰[1] 杨宝峰[1] 

机构地区：[1]哈尔滨医科大学药理学教研室黑龙江省生物医药重点实验室省部共建国家重点实验室培育基地,黑龙江哈尔滨150081

出　　处：《哈尔滨医科大学学报》2009年第3期211-214,共4页Journal of Harbin Medical University

基　　金：2006年度国家博士点基金项目(20060226019);黑龙江省科学技术攻关重点项目(2006G2215-00)

摘　　要：目的探讨犬心房纤颤(atrial fibrillation,AF)模型中微囊蛋白-3(Caveolin-3)蛋白表达情况。方法建立犬房颤(AF)模型,通过免疫荧光染色和Western blot技术检测心房快速起搏建立的犬AF模型及AF患者心房肌的Caveolin-3蛋白表达情况。结果在犬和人房颤的心房组织,Western blot结果显示Caveolin-3蛋白表达明显降低,与非房颤组织相比P<0.05。在犬假手术组和窦性心律的患者,免疫荧光结果显示位于心房组织细胞膜上Caveolin-3蛋白表达明显,并均匀分布在细胞膜表面;而在AF状态下,人和犬心房肌Caveolin-3蛋白表达显著降低。结论Caveolin-3参与了AF这一临床复杂病症的发生和发展。Objective To investigate the expression of Caveolin-3 in canine model of atrial fibrillation （AF） and patients with AF. Methods Model of atrial fibrillation was estabhshed. Immunofluorescence and Western blot were used to determine the expression of Caveolin-3 in the tissues with and without AF. Results Both immunofluorescenee and Western blot showed the expression of Caveolin-3 was significantly decreased in AF tissues compared to that in the normal groups （ P 〈 0. 05 ）. Immunofluoreseenee results of both canine group with sham-operation and patients with sinus rhythm showed that Caveolin-3 expressed obviously in the cell membrane of atria and distributed uniformly. However, expression of Caveolin-3 was significantly decreased in AF. Conclusion Caveolin-3 may be involved in the oceurance and development of AF.

关 键 词：心房纤颤 微囊蛋白-3 蛋白表达 

分 类 号：R332[医药卫生—人体生理学] R541.75[医药卫生—基础医学]