超顺磁性氧化铁标记脂肪干细胞移植入大鼠心脏后的活体磁共振示踪成像  

In vivo Magnetic Resonance Imaging Tracking of Transplanted Adipose-derived Stem Cells Labeled with Superparamagnetic Iron Oxide in Rat Hearts

作　　者：刘再毅[1,2] 王瑛[3] 王广谊[1,2] 李晓红[2] 李晏[1,2] 粱长虹[1,2] 

机构地区：[1]广东省人民医院放射科,广州510080 [2]广东省医学科学院医学影像部,广州510080 [3]广州医学院附属第一医院超声科,广州510120

出　　处：《中国医学科学院学报》2009年第2期187-191,I0010,共6页Acta Academiae Medicinae Sinicae

摘　　要：目的探讨超顺磁性氧化铁(SPIO)标记脂肪干细胞(ADSCs)移植入大鼠心脏后的活体磁共振成像(MRI)示踪的可行性。方法使用左旋多聚赖氨酸-SPIO共培养方式标记ADSCs。采用普鲁士蓝染色及透射电镜观察细胞内铁颗粒,台盼兰染色检测细胞活力,并对标记的干细胞进行体外MRI成像。将经过SPIO标记的干细胞移植入正常大鼠心脏,使用MRI进行活体成像观察并与病理结果进行对照分析。结果普鲁士蓝染色于ADSCs胞浆内可见蓝色颗粒,电镜检查见铁颗粒位于内涵体/溶酶体内;台盼兰染色检测细胞活力实验组与对照组差异无显著性(P>0.05);标记了SPIO的干细胞体外MRI成像时,实验组信号显著低于阴性对照组和空白对照组;标记后的干细胞移植到心脏后,MRI显示细胞移植区域信号缺失,对应区域病理切片普鲁士蓝染色可见胞浆内染色阳性的细胞。结论MRI可以对移植入心脏的经SPIO标记的干细胞进行无创、动态的活体示踪成像,有助于了解移植细胞在体内的存活及迁徙情况。Objective To investigate the feasibility of in vivo magnetic resonance imaging （MRI） tracking of transplanted adipose-derived stem cells （ADSCs） labeled with superparamagnetic iron oxide （SPIO） in rat heart. Methods ADSCs were labeled with poly-L-lysine （PLL） -SPIO complexes. Intracellular iron uptake was identified by Prussian blue stain and transmission electromicroscopy. Trypan blue staining was used to test the viability of the labeled cells. In vitro MRI of labeled ceils was performed. SPIO-labeled ADSCs were transplanted into normal rat hearts and were in vivo imaged with MRI. Image findings on MRI were correlated with histological findings of the rat hearts. Results The labeling efficacy of ADSCs with PLL-SPIO was nearly 100%. Light microscopy revealed the SPIO particles were located in the cytoplasm of the ADSCs by Prussian blue staining. Transmission electromicroscopy revealed that the SPIO particles were located in the endosomes in the cytoplasm. There was no significantly deference in viability between labeled and unlabeled groups demonstrated by Trypan blue test （ P 〉 0. 05 ）. MRI showed signal loss in gel mixed with labeled cells as compared with the unlabeled cells group and blank group. Signal void on rat hearts were demonstrated on MRI and were well correlated with histological findings where Prussian-blue-stain positive cells presented. Conclusion MRI can be used to in vivo track the transplanted ADSCs labeled with SPIO into rat hearts and facilitate to understand the conditions of the labeled ceils in the transplanted areas.

关 键 词：干细胞移植 磁共振成像 活体示踪 超顺磁性氧化铁 标记 

分 类 号：R445.2[医药卫生—影像医学与核医学]