c-kit在自体中厚移植皮片中的表达  

EXPRESSION OF c-kit IN HUMAN INTERMEDIATE-SPLIT-THICKNESS SKIN GRAFTS

作　　者：曹芳[1,2] 薛春雨[3] 邢新[3] 

机构地区：[1]无锡市第二人民医院整形外科,江苏无锡214001 [2]上海交通大学医学院附属第九人民医院整复外科 [3]第二军医大学附属长海医院整形外科

出　　处：《中国修复重建外科杂志》2009年第7期814-817,共4页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：国家自然科学基金资助项目(30371473)~~

摘　　要：目的检测c-kit在人自体移植中厚皮片中与正常皮肤中的表达差异,初步探讨c-kit在自体移植皮片色素沉着发生机制中的作用。方法取患者自愿捐赠面颈部自体中厚皮片移植术后1年的色素沉着皮片16例,以自体移植皮片受区周围及原供区皮肤作为对照。采用Envision免疫组织化学染色方法,检测c-kit在各皮片的表达与分布;使用医学图像定量分析系统定量检测各标本中c-kit蛋白的阳性染色指数(positive index,PI)。结果c-kit的表达定位于表皮基底部黑素细胞、角朊细胞胞浆或细胞膜表面及真皮中的腺体上皮细胞。c-kit在大部分自体中厚移植皮片中呈强阳性表达,在表皮基底层处形成棕褐色条带;在受区周围正常皮肤及原供区周围皮肤中有局灶性阳性表达。c-kit在自体移植中厚皮片、受区周围及原供区周围正常皮肤中的PI值分别为131216±19130、92958±16208、91306±8135,自体移植中厚皮片c-kit表达与周围皮肤比较差异均有统计学意义(P<0.05)。结论c-kit在自体中厚移植皮片中的表达较自体正常皮肤中显著增高,在自体移植皮片过度色素沉着中起重要调控作用。Objective To investigate the expression of c-kit in human intermediate-split-thickness skin grafts and normal skin, and to recognize the role of c-kit in hyperpigmented process of the skin grafts. Methods The hyperpigmented intermediate-split-thickness skin grafts of 16 patients＇ face and cervicum 1 year after operation was harvested and the normal skins around the recipient site and the donor site were used as controls. Envision immunohistochemical technique was used to detect the expression and distribution of c-kit in the skin grafts and in the normal control skins, respectively. Medical image quantitative analysis system was used to quantitate the positive expression index （PI）. Results The expression of c-kit was located in endochylema and cytolemma of melanocytes and keratinocytes in the basilar part of epidermis. The positive expression of c-kit was obvious in the intermediate-split-thickness skin grafts and blown zone was observed in the basilar part of epidermis; and was not obvious and local in normal control skins. The PI of c-kit in the intermediate-spl it-thickness skin grafts （131 216 ± 19 130） was significantly higher than that in the normal skin around the recipient site （92 958 ± 16 208） and in the normal skin around the donor site （91 306 ± 8 135）; showing statistically significant difference （P 〈 0.05）. Conclusion The expression of c-kit in intermediate-split-thickness skin grafts increases remarkably compared with that in normal control skin. c-kit may play an important adjusting role in the process of hyperpigmentation of skin grafts.

关 键 词：C-KIT 中厚移植皮片 色素沉着 

分 类 号：R622[医药卫生—整形外科]