猪体细胞克隆技术的优化  

Optimization of Somatic Cell Nuclear Transfer in Pigs

作　　者：张德福[1,2] 戴建军[1,2] 吴彩凤[1,2] 吴华莉[1,2] 刘东[1,2] 陈学进[3] 陈莹[3] 王凯[3] 

机构地区：[1]上海市农业科学院畜牧兽医研究所,上海201106 [2]上海市农业遗传育种重点实验室,上海201106 [3]上海交通大学医学院附属新华医院发育生物学实验室,上海200092

出　　处：《农业生物技术学报》2009年第3期365-369,共5页Journal of Agricultural Biotechnology

基　　金：上海市科技兴农重点攻关项目(沪农科攻字[2003]第14-1号和沪农科攻字[2005]第3-5号);上海市科技兴农推广项目(沪农科推字2007-3-7号);上海市科学技术发展基金(No.014909005,04DZ05611);德国BMBF(No.CHN99/310)基金

摘　　要：用体细胞核移植方法生产的克隆猪诞生至今已经8年,虽然同胚胎细胞核移植相比,体细胞核移植技术难度大,成功率低（1%-2%）,但近年来用体细胞核移植技术克隆猪有了较深入的研究,在基本机理和关键技术上取得了一定的进展。结合本实验室近年来的相关研究工作,综述了用核移植方法生产体细胞克隆猪的技术关键和难点及应用情况,并对提高猪体细胞核移植效率的策略进行了分析。It has been almost eight years since the live piglets produced by somatic cell nuclear transfer （SCNT） technology was first reported. Although it is more difficult and low efficiency （1%-2%） compared with embryonic cell nuclear （ECNT） technology,the cloning pigs with SCNT technology has been gotten further studied, and some progress has been taken in its mechanism and approaches. In this article, the key technology and difficulties of SCNT in cloning pigs, including applications and approach of increasing the efficiency of SCNT was mainly reviewed.

关 键 词：猪 猪体细胞核移植 优化 

分 类 号：S814.8[农业科学—畜牧学] TQ460.38[农业科学—畜牧兽医]