猪应激性状QTL微卫星标记与运输应激后肉质性状关系的研究  

Effects of Microsatellite DNA Markers in Stress QTL Regions on Meat Quality in Responses to Transport

作　　者：王晓娜[1] 汪秀星[1] 赵茹茜[2] 刘香[1] 薛春阳[1] 陈杰[1,2] 

机构地区：[1]南京农业大学动物科技学院,南京210095 [2]南京农业大学动物医学院,农业部动物生理生化重点开放实验室,南京210095

出　　处：《农业生物技术学报》2009年第3期386-392,共7页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金重点项目(No.30430420)资助

摘　　要：在应激敏感品种皮特兰猪和抗应激品种二花脸猪为亲本建立的F2资源群体中选取52头140-150日龄、体重为65±5kg的猪。2h运输应激试验后静脉注射巴比妥钠麻醉屠宰取样。在已知的应激性状QTL座位区域内选择了22个微卫星标记,对各微卫星标记与运输应激后的pH45min、pH24h、嫩度、系水力、蒸煮损失以及肉色评分（1^＊,a^＊,b^＊）6项肉质指标进行了相关性分析。结果表明,各位点等位基因数为3-8个,杂合度为0.4155-0.7432,多态信息含量为0.3651-0.8150。方差分析显示,S0101对肉色a＊有极显著影响（P〈0.01）;SW1023对系水力有显著影响（P〈0.05）;SW1808对系水力有极显著影响（P〈0.01）,对肉色1^＊和a^＊有显著影响（P〈0.05）;S0112和S0092对pH45min有显著影响（P〈0.05）。研究结果表明在应激QTL座位内选取的这5个微卫星标记对运输后的猪肉品质存在显著影响。The effects of 22 microsatellite DNA loci on meat quality related to stress following transport trial in pigs were analyzed. These subjects were selected from the segregating F2 generation of a Pietrain （PIE）（stress sensibility）×Erhualian （EHL）（stress tolerance） cross, pH45~an, pH24h, tenderness, water holding capacity, cooking loss, and color scores- 1^＊, a^＊, b^＊ of the pork were measured after the transport of 2 hours. One-way ANOVA was applied to analyze the relation between microsatellite polymorphisms and meat quality. The results revealed that the allelic number was 3-8, heterozygosity was 0.42-0.74 and polymorphism information content was 0.37-0.82. S0101 had significant effect on meat color-a^＊ （P 〈0.01）. The effect of SW1023 on water holding capacity （WHC） was significant （P 〈0.05）. The effects of SW1808 on WHC （P 〈0.01）, meat color-l＊（P 〈0.05） and b^＊（P 〈0.05） were significant, respectively. Both S0112 and S0092 had significant effects on pH45min （P 〈0.05）. Result suggested that these 5 microsatellites in swine stress QTL regions were closely associated with meat quality in response to transport.

关 键 词：F2群体 微卫星 运输应激 肉质性状 相关分析 

分 类 号：S188[农业科学—农业基础科学] S185