肌肉生成抑制素和抑制素融合表达质粒的构建及其表达  

Construction and Expression of Recombinant Plasmid Co-expressing Myostatin and Inhibin Genes

作　　者：张路培[1] 杨润军[2] 甘乾福[2] 许尚忠[1,2] 李俊雅[1] 高雪[1] 任红艳[1] 陈金宝[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所,北京100193 [2]西北农林科技大学动物科技学院,杨凌712100

出　　处：《农业生物技术学报》2009年第3期409-412,共4页Journal of Agricultural Biotechnology

基　　金：国家农业科技支撑计划项目(No.2006BAD01A10和No.2006BAD14B07)资助

摘　　要：以乙肝表面抗原S基因为载体,分别将肌肉生成抑制素（myostatin）N端基因片段（MSTN）和抑制素（inhibin）N端基因片段（INH）分别插入S基因编码的C端和第112~113氨基酸残基密码子之间,构建MSTN-INH融合表达质粒pVAX-SMI。酶切和测序鉴定表明,重组质粒pVAX-SMI构建成功。脂质体法将pVAX-SMI转染HeLa细胞,Westernblot检测重组蛋白的表达,结果表明,重组蛋白成功在HeLa细胞中表达。Two DNA fragments encoding N-term ofmyostatin （MSTN） and inhibin （IHN） were incorporated into Hepatitis B virus S gene at C-term and site of amino acid sequence 112 and 113 to construct an expression plasmid of pVAX-SML The plasmid pVAX-SMI was identified by restriction endonuclease digestion and sequencing, pVAX-SMI was then transfected into HeLa cells and the recombinant protein was detected by Western blot. The result showed that the plasmid could express recombinant MSTN and/NH successfully. And the pVAX-SMI expression plasmid was constructed successfully.

关 键 词：肌肉生成抑制素 抑制素 基因表达 乙肝表面抗原 

分 类 号：S188[农业科学—农业基础科学]