芸薹属A基因组DNA封阻下的甘蓝C染色体组核型分析  

Karyotype Analysis of Brassica oleracea C Genome Based on Brassica A Genomic DNA as Block Reagent

作　　者：陈晓丹[1] 朱利泉[1] 王永[1] 荣小营[1] 卢军[1] 王小佳[1] 

机构地区：[1]西南大学农学与生物科技学院,重庆400716

出　　处：《农业生物技术学报》2009年第3期504-509,共6页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(No.30671429);重庆市自然科学基金(No.9266)资助

摘　　要：为探索一种高效可靠的芸薹属植物核型分析方法,以甘蓝(Brassica oleracea)和白菜(B.pekinensis)为材料,提取甘蓝基因组DNA用地高辛标记为探针,提取白菜基因组DNA作为封阻剂,对甘蓝有丝分裂中期染色体进行原位杂交。结果表明,甘蓝每对同源染色体上均显示出了特定的原位杂交信号,依据信号特征成功地将甘蓝9对染色体进行了精细的分型。为甘蓝等小型染色体的核型分析提供了一条可行且精确的方法。To explore an effective and reliable karyptyping method in Brassica crop plants, karyptype of Brassica oleracea was conducted successfully by genomic in situ hybridization using B.oleracea genomic DNA as probe labeled with DIG-High Prime Mix Kit and B.pekinensis genomic DNA as block reagent. The results indicated that specific fluorescent signals were shown on each pair of homologous chromosome and nine pairs chromosomes of B.oleracea were exactly identified according to signal characteristics. It provided a feasible and accurate method to karyptype analysis of small chromosomes.

关 键 词：甘蓝 白菜 基因组原位杂交 核型分析 

分 类 号：S188[农业科学—农业基础科学]