川桂叶中黄酮类化合物的提取与测定  

Extraction and Determination of Flavonoids from Cinnamomum Wilsonii Gamble Leaves

作　　者：李姣娟[1] 黄克瀛[2] 龚建良[2] 周尽花[1] 陈丛瑾[2] 

机构地区：[1]中南林业科技大学应用化学研究所,长沙市韶山南路498号410004 [2]中南林业科技大学材料科学与工程学院,长沙市410004

出　　处：《光谱实验室》2009年第3期530-534,共5页Chinese Journal of Spectroscopy Laboratory

基　　金：湖南省教育厅基金资助项目(07C811)

摘　　要：采用加热回流法提取川桂叶中的黄酮类化合物,建立了川桂叶中总黄酮含量测定的光谱分析方法。结果表明:以芦丁为标准品,用NaNO2-Al(NO3)3-NaOH显色法可测定川桂叶中总黄酮的含量,该方法的精密度和准确度均较高,相对标准偏差为0.63%,方法的回收率为97.18%—102.20%。通过正交试验优化,得到提取川桂叶中黄酮类化合物的最佳工艺条件是:用体积分数为70%的乙醇作溶剂,在固液比1∶20(g/mL)、提取温度80℃的条件下提取1.5h,连续提取2次。测得川桂叶中总黄酮含量为22.53mg·g-1。Flavonoids were extracted from Cinnamomum wilsonii Gamble leaves by Heating refluxing, and determined by spectrophotomertry. The NaNO3-Al （NO3）2-NaOH method is practicable to determine flavonoids content from C. wilsonii Gamble leaves with rutin as the standard. This method is higher precision and accuracy with RSD 0. 63% and recoveries 97. 18%-102.20%. The optimum extraction conditions were obtained by orthogonal experiments as follows：extracting solvent 70% （V/V） ethanol, material ratio 1 ： 20 （g/mL）, extraction temperature 80℃, extraction duration 1.5h and extracting for two times. The total flavonoids content from C. wilsonii Gamble leaves is 22. 53mg · g^-1.

关 键 词：川桂叶 黄酮类化合物 提取 分光光度法 

分 类 号：O657.32[理学—分析化学]