积雪草总黄酮提取工艺和含量测定研究  

Extracting Technology and Determination of Total Flavonoids in Centella Asiatica

作　　者：王荣[1] 黎云祥[1] 蔡凌云[1,2] 高侠[1] 

机构地区：[1]西华师范大学生命科学学院四川省高校环境科学与生物多样性保护重点实验室,四川省南充市师大路1号637002 [2]凯里学院化学系,贵州省凯里市文化北路22号556000

出　　处：《光谱实验室》2009年第3期544-549,共6页Chinese Journal of Spectroscopy Laboratory

基　　金：四川省重点学科项目(SZD0420)资助;四川省教育厅重点项目(2006A078)

摘　　要：确定积雪草总黄酮最佳提取工艺和含量测定方法。采用微波辅助法提取积雪草中的黄酮类化合物,通过正交实验确定了最佳提取工艺。分别以槲皮素和山奈酚为对照,采用紫外分光光度法在320nm处测定积雪草全草中的总黄酮。最佳工艺条件:提取次数3次,辐射时间20s,固液比1∶10,功率480W。按最佳工艺条件提取总黄酮含量为8.40mg/g。回归方程为(槲皮素):y=29.993C+0.027,相关系数r2=0.9982,精密度和稳定性试验RSD分别为0.265%和0.172%(n≥6),平均加样回收率为99%,RSD=1.51%(n=5)。回归方程为(山奈酚):y=36.928C+0.0178,相关系数r2=0.9991,精密度和稳定性试验的RSD分别为0.231%和0.190%(n≥6),平均加样回收率为99%,RSD=0.714%(n=5),样品的稳定性试验RSD为0.218%(60min)。选择的最佳工艺对提取积雪草全草总黄酮确为最佳。用槲皮素或山奈酚标准品,采用紫外分光光度法,操作简便,结果准确,灵敏度高,重复性好,为积雪草全草中总黄酮含量测定的一种切实可行的方法。Microwave-assisted extraction of total flavonoids in leaves of centella asiatica, the optimum technology parameters were obtained by orthogonal tests. Total flavonoids in centella asiatica were determined at 320nm by ultraviolet spectrophotometry with quereetin and kaempferol as standard control. The result shows the follwing the optimal extraction conditions：intermittent time was 3, MAE time 20s , solid/liquid ratio 1 ： 10, and applied power 480W. under the optimal conditions,the content of total flavonoids was 8.40mg/g. The regression equation with quercetin was y = 29. 993C ＋ 0. 027, r^2 = 0. 9982, the precisions： RSD = 0. 265 %, RSD = 0. 172 % （n ≥ 6）, the average recovery of flavonoids was 99%, RSD = 1. 51%（n = 5）. The regression equation with kaempferol was y=36. 928Cq-0. 0178,rZ=0. 9991,the precisions：RSD =0. 231%,RSD =0. 190% （n= 6）, the average recovery of flavonoids was 99%, RSD=0. 714% （n=5） , stability test of the sample, RSD was 0. 218% （in 60min）. The method is highly sensitive and reproducible and can be used as a quantitative analysis method for total flavonoids in Centella asiatica.

关 键 词：积雪草 总黄酮 微波辅助提取 紫外分光光度法 正交实验 

分 类 号：O657.32[理学—分析化学]