VP2蛋白Cys-402、Cys-214对猪细小病毒VLPs影响的初步研究  

A primary study on effects of Cys-402 and Cys-214 of VP2 protein on PPV VLPs

作　　者：徐志文[1] 胡秋炅[1,2] 郭万柱[1] 朱玲[1] 王印[1] 石恬[1] 王小玉[1] 

机构地区：[1]四川农业大学动物医学生物技术中心,四川雅安625014 [2]乐山市畜牧食品局,四川乐山630000

出　　处：《黑龙江畜牧兽医》2009年第7期17-19,共3页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(30500019);四川省科技攻关项目(05NG002-011);四川省青年科技基金项目(2006Q14-049)

摘　　要：试验以猪细小病毒VP2 Cys-402（A）、Cys-214（B）定点突变体为基础，将突变体真核表达载体转染COS-7细胞及核酸免疫猪细小病毒抗体阴性的小白鼠，通过电镜技术和流式细胞仪技术探讨突变Cys-402、Cys-214对猪细小病毒病毒样颗粒的影响，旨在寻找利于外源基因插入的病毒样颗粒内部位点。电镜和小白鼠核酸免疫试验结果表明：Cys-214对病毒样颗粒的组装和免疫原性的发挥是必需的，其突变后不能促使VP2形成病毒样颗粒，更不能在免疫后的小白鼠血清中监测出相应抗体的存在；而Cys-402的突变并不干扰病毒样颗粒的形成和免疫原性的发挥。由此可以推测Cys-402及其附近可能存在利于外源基因插入的位点。Based on the porcine parvovirus（PPV） VP2 mumants, they were transfeeted into COS -7 cells, and were injected into mice as nu- cleinic acid vaccine. The purpose of this article is to study the effects of Cys -402 and Cys -214 of VP2 an PPV VLPs and to find the sites in the VLPs which are profit to exogenous gens insertion. The result of electron microscope and Balb/c mice immunity test displayed that Cys -214 played an important role on VLPs＇ formation and immunogenicity; inversely, the mutation of Cys -402 was not to produce a marked effect on VLPs＇ formation and immunogenicity. It was supposed that there was site which was benefit to exogenous gene insertion on Cys -402 or nearby. The study has provided theory reference to muhivalent vaccine and antigen transport system which were based on PPV VLPs

关 键 词：猪细小病毒 VP2 病毒样颗粒 定点突变 

分 类 号：S852.659.2[农业科学—基础兽医学]