怀地黄SRAP分子标记体系的建立与DNA指纹图谱的构建  

Optimization of Amplification and Construction of DNA Fingerprinting for Rehmannia Glutinosa Libosh. f. Hueichingensis (Chao et Schih) Hsiao Based on SRAP

作　　者：谷凤平[1] 周春娥[1] 路淑霞[1] 姚换灵[1] 王芳[1] 段红英[1] 周延清[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007

出　　处：《河南师范大学学报（自然科学版）》2009年第3期175-178,共4页Journal of Henan Normal University(Natural Science Edition)

基　　金：国家高技术研究发展计划(863)项目子课题(2006AA100109)资助

摘　　要：以怀地黄DNA为模板,进行SRAP反应条件的优化及DNA指纹图谱构建,优化的反应体系为:25μL的体积中,模板DNA 20 ng,Mg2+浓度2.5 mmol.L-1,上下游引物各0.36μmol.L-1,dNTPs 0.30 mmol.L-1,Taq DNA酶2.0 U.构建了怀地黄23个品种的DNA指纹图谱,为怀地黄品种鉴定、遗传多样性分析、分子标记辅助育种和质量综合评价等研究奠定了基础.In the present study,SRAP-PCR parameters are optimized using Rehmannia Glutinosa Libosh. f. Hueichingensis （Chao et Schih） Hsiao,DNA as template. SRAP fingerprinting for different cultivars of Rehmannia glutinosa L. is contructed. The optional parameters for 25μL PCR reaction volume are as follows： Mg^2＋ 2.5 mmol·L^-1 ,dNTPs 0.30 mmol·L^-1 , primer0. 36 μmol·L^-1 , DNA polymerase 2. 0 U,and template DNA 20 ng. SRAP-PCR amplification by which SRAP DNA fingerprinting for different cultivars of Rehmannia glutinosa L. is successfully constructed,This is the foundation for the cultivar identification, genetic diversity evaluationmarker-assisted selection breeding and quality assessment.

关 键 词：怀地黄 SRAP扩增体系优化 SRAP DNA指纹图谱 

分 类 号：Q341[生物学—遗传学]