Omi／HtrA2在窒息后血清诱导人近曲肾小管上皮细胞凋亡中的作用  

Role of Omi/HtrA2 in renal tubular cells apoptosis induced by postasphyxial serum of neonate

作　　者：张勇[1] 董文斌[1] 李清平[1] 邓存良[2] 熊涛[1] 雷小平[1] 郭琳[1] 

机构地区：[1]泸州医学院附属医院新生儿科,四川泸州646000 [2]泸州医学院附属医院传染与免疫研究室,四川泸州646000

出　　处：《中国危重病急救医学》2009年第6期346-348,I0001,共4页Chinese Critical Care Medicine

基　　金：四川省杰出青年学科带头人培养基金项目（04ZQ026-033）;四川省科技厅应用基础项目（2008JY0015）

摘　　要：目的研究Omi／HtrA2在新生儿窒息后血清诱导人近曲肾小管上皮细胞（HK-2）凋亡时的作用机制。方法以HK-2细胞为研究对象，实验分为空白对照组、窒息组、Ucf-101（Omi／HtrA2的特异性阻断剂）干预组。以体积分数为20％的窒息后24h血清作为攻击血清，以终浓度为10μmol／L的Ucf-101对窒息组进行干预。用激光共聚焦显微镜观测Omi／HtrA2在胞内的转位状况，采用流式细胞仪检测各组细胞凋亡率。结果窒息血清攻击后，Omi／HtrA2由线粒体转位到胞质中，阳性转位细胞率较空白对照组明显增加[（28．1±3．6）％比（9．4±2．1）％，P〈0．01]。与空白对照组比较，窒息组细胞凋亡率明显增加（（36．3±4．4）％比（12．4±2．9）％，P〈0．01]。与窒息组比较，干预组细胞凋亡率明显减少[（27．0±3．9）%比（36．3±4．4）％，P〈0．01]。结论窒息后新生儿血清诱导HK-2细胞凋亡，Omi／HtrA2由线粒体转位到线粒体外，在其胞内凋亡信号转导过程中占有重要作用。Objective To investigate the mechanism of inducing apoptosis of Omi/HtrA2 in renal tubular cells with postasphyxial serum of neonate. Methods Human renal proximal tubular cell line HK-2 cell was used as target cell. They were divided into three groups： control group, asphyxia group and Ucf-101 （Omi/HtrA2 special inhibitor） treated group. The challenge concentration of serum obtained from neonates 24 hours after asphyxia was 20%, and the treatment concentration of Ucf-101 was 10 μmol/L. The Omi/HtrA2 translocation in renal tubular cells was observed with confocal microscopy, and the rate of apoptosis was detected with flow cytometer. Results It was found that Omi/HtrA2 was translocated into cytoplasm in asphyxia group, and the rate of Omi/HtrA2 translocation in HK-2 cells of asphyxia group was significantly increased [（28.1±3.6） % vs. （9.4±2.1 ）%, P〈0.01]. Compared with the control group, after being treated with postasphyxial serum, the rate of apoptosis of HK-2 cells in asphyxia group was significantly increased [（36.3±4.4）% vs. （12.4±2.9）%, P〈0.01]. Compared with asphyxia group, the rate of apoptosis in HK 2 cells in Uef-101 treated group was significantly decreased [（27.0±3.9）% vs. （36.3±4.4） %, P〈0. 01]. Conclusion These experimental data demonstrates that postasphyxial serum of neonate can induce apoptosis of HK 2 cells, and translocation of Omi/HtrA2 from mitoehondria into cytoplasm may play an important role in its intracellular signal transduction mechanism in induction of apoptosis.

关 键 词：新生儿 窒息 血清 OMI/HTRA2 转位 

分 类 号：R686[医药卫生—骨科学]