亚精胺预处理对NaCl胁迫下青稞幼苗生理特性的影响  

The Effects of Spermidine Pretreatment on Physiological Characteristics of Hulless Barley Seedlings under NaCl Stress

作　　者：段辉国[1,2] 赵俊茗[3] 张轩波[1,2] 丁晓波[2] 杨汉波[2] 谭春华[2] 

机构地区：[1]四川省高等学校特色农业资源研究与利用重点实验室,四川内江641112 [2]内江师范学院化学与生命科学学院,四川内江641112 [3]四川农业大学水稻研究所,四川温江611130

出　　处：《西北植物学报》2009年第6期1220-1225,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：四川省科技厅应用基础项目(05JY0292017);内江师范学院生态学重点建设学科项目

摘　　要：以青稞幼苗为试验材料,通过水培实验研究了外源亚精胺(Spd)预处理对200mmol·L-1NaCl盐胁迫下青稞幼苗叶片相对含水量(RWC)、光合色素、水溶性蛋白质、丙二醛和脯氨酸含量与电解质外渗率的影响,探讨外源亚精胺提高青稞幼苗抗盐胁迫能力的机理.结果表明:随着盐胁迫的延续,单独NaCl处理组和NaCl+Spd处理组的青稞幼苗叶片RWC、光合色素与水溶性蛋白质含量均明显持续降低且显著低于同期对照,而丙二醛含量、电解质外渗率则逐渐增加且显著高于同期对照,Spd预处理则显著延缓了这些指标的变化幅度;同时上述两组处理也均使青稞幼苗脯氨酸含量不断增加且显著高于同期对照,Spd预处理则增加幅度更大.可见,Spd预处理缓解了青稞幼苗叶片失水程度和光合色素、蛋白质含量下降幅度,提高了质膜的稳定性和完整性,增加了渗透调节物质脯氨酸含量,从而减轻了NaCl胁迫对青稞幼苗造成的伤害,最终提高青稞幼苗抗盐胁迫能力.The effects of spermidine （Spd） pretreatment on relative water content （RWC）,levels of photosynthetic pigment,soluble protein, malonyldialdehyde （MDA）, proline and electrolyte leakage in leaves of hulless barley seedlings under 200 mmol·L^-1 NaCl stress were investigated. The results showed that RWC,the contents of photosynthetic pigment and soluble protein reduced constantly,MDA content and electrolyte leakage increased in seedling leaves under NaC1 stress,while these changes deferred in Spd pretreatment seedling. NaCl stress induced significantly increase of proline content, while Spd pretreatment accumulated more proline in seedling. The results suggested that Spd pretreatment could relieve water loss, damages of photosynthetic pigment, decrease of protein content in leaves and improve plasmalemma stability and integrity,increase proline content and alleviate damages by NaCI stress and finally promote salt resistance of hulless barley seedlings under NaCl stress.

关 键 词：亚精胺 NACL胁迫 青稞幼苗 生理特性 

分 类 号：Q945.78[生物学—植物学]