百合知母汤对自然衰老型围绝经期综合征肾虚证大鼠中枢神经递质含量的影响  

Effect of bai-he-zhi-mu-tang on the level of central nervoustransmitter in hypothalamus of natural-aging Shenxu zheng climacteric syndrome rats

作　　者：王华富[1] 蔡宝昌[1,2] 高钦[1] 崔姣[1] 陈敏敏[1] 许惠琴[1] 

机构地区：[1]南京中医药大学药学院,南京210046 [2]江苏省中药炮制重点实验室,南京210046

出　　处：《江西中医学院学报》2009年第3期53-55,共3页Journal of Jiangxi College of Traditional Chinese Medicine

基　　金：国家自然科学基金(C03050105)

摘　　要：目的:观察百合知母汤(BHZMT)对自然衰老型围绝经期综合征肾虚证大鼠脑内中枢神经递质含量的影响。方法:采用自然衰老雌性15～16月龄SD大鼠,随机分为:围绝经期组、围绝经期+BHZMT组、围绝经期+TX肾阴虚组、围绝经期+TX+BHZMT组、围绝经期+ADE肾阳虚组、围绝经期+ADE+BHZMT组,另设一青年对照组共7组,造模1周后,各组连续灌胃给予BHZMT3周。于末次给药次日,脱颈椎处死大鼠,迅速分离下丘脑,分别采用高效液相色谱——电化学方法测定单胺类神经递质NE、DA、5-HT的含量和免疫组化方法测定P物质和β-内啡肽的含量。结果:围绝经期大鼠NE、β-内啡肽水平下降,DA、5-HT、P物质水平明显升高;肾阴虚证大鼠NE、DA、5-HT、P物质水平明显升高,β-内啡肽则明显减少;肾阳虚证大鼠5-HT水平明显升高;经BHZMT治疗后,肾阴虚大鼠下丘脑NE、DA、5-HT(P<0.01)、P物质的含量降低,β-内啡肽的含量升高;肾阳虚大鼠下丘脑NE、DA含量升高,5-HT水平降低,但无显著性差异。结论:BHZMT可有效调节围绝经期肾阴虚大鼠脑内中枢神经递质含量,从而改善围绝经期综合征。Objective： To observe bai-he-zhi-mu-tang （BHZMT） on the level of the central nervous transmitter in hypothalamus of natural-aging Shenxu Zheng climacteric syndrome rats. Method： 15-16 month old female SD rats are divided randomly into 6 group： Climacteric, Climacteric ＋ BHZMT, Climacteric ＋ TX Shenyingxu Zheng, Climacteric ＋ TX ＋ BHZMT, Climacteric ＋ ADH Shenyangxu Zheng, Climacteric ＋ ADH ＋ BHZMT,then set a youth group with three-month old female SD rats. one week later,we begin to ig BHZMT for three weeks, At the end of the meeting the next day delivery, Death from cervical rats, Rapid separation of the hypothalamus, We detect the level of NE, DA and 5-HT in hypothalamus by using HPLC with electrochemical detection and the level of SP, β-EP by using immunohistochemical method. Result ： Compared to normal group, the level of NE, β-EP reduce, DA,5-HT, SP increased significantly in climacteric syndrome rats, the level of NE, DA,5-HT, SP increased significantly, the level of β-EP reduced in Shenyingxu Zheng rats, the level of 5-HT increased significantly in Shenyangxu Zheng rats. After treatment of BHZMT, Shenyingxu Zheng group NE, DA ,5-HT （P 〈 0.01 ）, SP reduce, β-EP levels rise, ShenyangXu-type group NE, DA increased, 5-HT reduce, but no significant difference. Conclusion： BHZMT can effectively regulate the content of central nervous transmitter of ShenyingXu-type climacteric rats rat SbenyiugXu-type rats of the central nervous transmitter content so as to improve the climacteric syndrome.

关 键 词：百合知母汤(BHZMT) 围绝经期综合征 肾虚证 中枢神经递质 

分 类 号：R285.5[医药卫生—中药学]