严重急性呼吸综合征肺水通道蛋白1表达的研究  

Expression of AQP1 in SARS and ARDS lungs

作　　者：张成[1] 王广发[1] 汤秀英[1] 李枫[2] 唐岩[2] 顾江[2] 

机构地区：[1]北京大学第一医院呼吸内科 [2]北京大学医学部组织学与胚胎学系,北京100034

出　　处：《中国实用内科杂志》2009年第7期617-619,共3页Chinese Journal of Practical Internal Medicine

基　　金：国家自然科学基金(30340030)

摘　　要：目的探讨水通道蛋白1(AQP1)参与严重急性呼吸综合征(SARS)肺水肿、急性肺损伤(ALI)的可能机制。方法2003年5月至6月运用免疫组织化学法对7例SARS死亡病例肺标本,6例具有ALI表现的非SARS死亡病例肺标本及4例无明显病变的肺组织标本进行检测。用图像分析比较三者表达量的不同,并结合临床资料探讨影响AQP1表达变化的因素。结果AQP1在正常人肺组织微血管内皮、肺泡上皮的胞膜上和胞浆中均有表达。AQP1在SARS肺和非SARS所致ALI肺中的表达均较正常肺组织明显下降(P<0.05);而SARS肺中AQP1的表达和非SARS所致ALI肺中AQP1的表达差异无统计学意义(P>0.05)。结论AQP1的表达下调可能参与了各种因素造成的ALI时肺水肿形成。与非SARS所致ALI相比,AQP1在SARS肺的表达改变无特异性。本研究为进一步探讨SARS肺水肿的形成机制及AQP1在生理和病理状态下的功能提供实验依据,也为临床治疗肺水肿提供了新的思路。Objective To invesigate the possible pathological mechanism of aquaporinl （ AQP1 ） in the formation of pulmonary edema and acute lung injury. Methods The expression of AQP1 in normal lung tissues, typical ARDS and SARS lung tissues were studied by immunohistochemistry with a mouse monoclonal antibody against AQP1 of human origin. An image analysis system was used to calculate the mean optical density（MOD） ,which represents its expression abundance. The differences of AQP1 expression among the three kinds of specimens were also compared. Results Immunolabelling showed AQP1 was stained both in microvascular endothelia and pulmonary alvolar epithelial cells. Image analysis indicated that AQP1 expression decreased significantly（P 〈 0.05 ） in both SARS and typical acute injured lungs compared to normal specimen. However no statistical difference existed between the lungs of SARS and typical ARDS. Conclusion The decreased expression of AQP1 in both SARS and typical ARDS lungs suggests that AQP1 may play a role in abnormal fluid transport in pathological conditions. Moreover, the changes of AQP1 expression in SARS lungs show no specificity in comparison with those in typical ARDS lungs.

关 键 词：水通道蛋白1 严重急性呼吸综合征 急性肺损伤 

分 类 号：R5[医药卫生—内科学]