原癌基因c-fos在大鼠缺血再灌注肾组织中的表达  

The expression of protooncogene cfos in ischemicreperfusion rat renal tissue

作　　者：程向明 刘平[1,2,3] 钟庆晨[1,2,3] 张留保 张爱羚 周其锦[1,2,3] 郭丹 沈爱云[1,2,3] 赵萍 

机构地区：[1]第二军医大学 [2]海军第414医院肾内科 [3]海军医学高等专科学校解剖组胚学教研室

出　　处：《第二军医大学学报》1998年第3期225-227,共3页Academic Journal of Second Military Medical University

基　　金：海军后勤部医药科研规划课题

摘　　要：目的：探讨原癌基因ｃ－ｆｏｓ在缺血再灌注肾脏中表达情况及其在急性缺血性肾脏损伤修复中的作用。方法：用免疫组织化学法及原位分子杂交技术，检测急性肾缺血再灌注后大鼠肾组织内Ｆｏｓ蛋白及ｃ－ｆｏｓｍＲＮＡ表达的分布、强度及时程动力学等指标。结果：缺血再灌注早期即可诱导肾组织中ｃ－ｆｏｓ的高效表达，且ｃ－ｆｏｓｍＲＮＡ表达早于Ｆｏｓ蛋白；Ｆｏｓ蛋白表达出现于再灌注后１ｈ，２～４ｈ达高峰，８ｈ开始锐减，２４ｈ消失；ｃ－ｆｏｓｍＲＮＡ再灌注后０．５ｈ出现表达，１ｈ达高峰，２ｈ开始锐减，４ｈ时几乎消失。Ｆｏｓ蛋白阳性分布主要集中于远曲小管、升支粗段及集合管细胞核内；ｃ－ｆｏｓｍＲＮＡ表达分布部位同Ｆｏｓ蛋白，但定位于细胞质内；而两者在肾小球内均无表达。结论：原癌基因ｃ－ｆｏｓ的高效表达可能参与缺血再灌注所致肾脏损伤修复的分子调控。Objective: To study the expression of protooncogene cfos in the ischemicreperfusion rat kidney and its recovery action in the acute ischemic kidney damage. Methods: The indexes of distribution, strength and time course of Fos protein and cfos mRNA expression in the ischemicreperfusion rat kidney were investigated by using immunohistochemistry and in situ hybridization. Results: (1) The expression of cfos gene in the rat kidney increased obviously in the early stage after ischemicreperfusion. Fos protein was first induced at 1 h,peaked at 2～4 h,decayed at 8 h and disappeared at 24 h after ischemicreperfusion. Expression of cfos mRNA appeared at 0.5 h,peaked at 1 h, decayed at 2 h,and disappeared nearly at 4 h after ischemicreperfusion. (2) Localization of Fos protein occurred predominantly in nuclei of the distal tubules,thick ascending limb and collecting duct cells . cfos mRNA appeared in cytoplasm of the same areas.Neither Fos protein nor cfos mRNA was seen in glomeruli. Conclusion: The results suggest that the high expression of protooncogene cfos might be involved in the molecular regulation mediating renal recovery from acute ischemia damage. The increase of cfos gene probably plays some roles in pathogenic process of acute renal ischemicreperfusion.

关 键 词：再灌注损伤 C-FOS基因 原位杂交 肾缺血 

分 类 号：R692.502[医药卫生—泌尿科学]