拟南芥干旱突变体远红外成像技术的筛选和特性鉴定  

Screening and Identification of Drought-Sensitive and-Insensitive Mutants of Arabidopsis thaliana by Infrared Thermography

作　　者：宋玉伟[1,2] 李玉英[1] 杨建伟[1] 

机构地区：[1]南阳师范学院生命科学与技术学院,南阳473061 [2]河南大学生命科学学院,开封475001

出　　处：《植物研究》2009年第4期466-470,共5页Bulletin of Botanical Research

基　　金：国家自然科学基金项目(编号:39870372,30370765和30440079)

摘　　要：利用化学诱变剂甲基磺酸乙酯(EMS)对模式植物拟南芥(Arabidopsis thaliana)进行化学诱变获得突变体筛选群体。在干旱胁迫下,以叶片的温度差异为筛选指标,利用远红外成像技术进行突变体的筛选,获得了对干旱不敏感突变体dri1(drought-insensitive1)和敏感突变体drs1(drought-sensitive1)。实验结果表明dri1和drs1为单基因隐性突变,气孔密度同野生型无差异,而叶片温度、气孔开度和叶片失水率则有明显改变。在MS培养基上的种子萌发实验表明在ABA、甘露醇和NaCl胁迫下dri1萌发率要比野生型高,而drs1则比野生型低。对突变基因的研究有待进一步进行。Drought-sensitive mutant （dril） and drought-insensitive mutant（ drsl ） of Arabidopsis thaliana were screened with infrared thermography from an ethyl methane sulfonate-mutagenized population. These mutants were identified as monogenic recessive mutations. Stomata density of these mutants was the same as that of wild type. While, their stomata apertures, water loss and leaf temperature were distinct compared with those of wild type under drought stress. In addition, seed germination test on MS medium indicated that dril was more insensitive to ABA, mannitol and NaC1 than the wild type, but drsl was not.

关 键 词：拟南芥 远红外成像技术 干旱 突变体 

分 类 号：Q945.78[生物学—植物学]