小鼠受精卵早期发育过程中Plk1表达和活性变化及其与MPF关系的研究  

Study the variation both xxpression and activity of Plk1 during early development of mouse zygote and its relation with MPF

作　　者：孙炳奇[1] 邱攀[1] 金明林[2] 于秉治[3] 

机构地区：[1]沈阳市第五人民医院,110023 [2]沈阳医学院基础医学院生物化学与分子生物学教研室 [3]中国医科大学基础医学院生物化学与分子生物学教研室

出　　处：《辽宁医学杂志》2009年第4期173-176,共4页Medical Journal of Liaoning

摘　　要：目的研究在小鼠受精卵早期发育过程中,哺乳动物Polo-like激酶(Plk1)的表达和活性变化及其与有丝分裂促进因子(MPF)活性变化的关系。方法以一细胞期小鼠受精卵为材料,用Western Blotting方法检测Plk1蛋白含量;用液闪计数分析仪分别测定Plk1和MPF的cpm值来表示各自激酶活性。结果小鼠受精卵第一次有丝分裂过程中Plk1的含量没有显著变化,而Plk1活性在M期达到最高峰,在M期退出时迅速下降。加入MPF抑制剂后MPF和Plk1活性均未在M期出现峰值。结论Plk1的活性是随着细胞周期的进程而变化的;Plk1在小鼠受精卵早期发育过程中可能参与了MPF的自我催化活化环。Objective To study the variation both expression and activity of Plkl and its relation with MPF during early development of mouse zygote. Methods The mouse zygote for research, to detect Plkl quantity by Western Blotling ; to detect Plkl and MPF activity by liquid Scintillation counters base on cpm count individual. Results The activity of PIkl attain to top at M phase and to decrease when exit M phase during the first cell cycle of mouse zygote. When MPF inhibitor added, neither MPF or Plkl activity attain to top at M phase. Conclusion The Plkl activity fluctuate to accompany cell cycle and Plkl maybe participate in the positive feedback loop that allows MPF autoamplification during early development of mouse zygote.

关 键 词：PLK1 MPF 受精卵 

分 类 号：R714.1[医药卫生—妇产科学]