大孔吸附树脂纯化当药中獐牙菜苦苷的研究  

Study on Purification of Swertiaman from Swertia pesudochinensis Hara. with Macroporous Adsorbent Resin

作　　者：周大成[1] 孟贺[1] 李荣生[2] 黄文哲[1] 秦文杰[1] 叶祖光[1] 

机构地区：[1]中药复方新药开发国家工程研究中心,北京100075 [2]中国中医科学院实验药厂,北京100700

出　　处：《中国中医药信息杂志》2009年第7期54-55,83,共3页Chinese Journal of Information on Traditional Chinese Medicine

摘　　要：目的确定纯化当药中獐牙菜苦苷的大孔吸附树脂的类型及工艺条件。方法以当药为原料,以当药中獐牙菜苦苷为指标,考察8种不同类型的大孔吸附树脂的纯化效果。结果HPD-100型大孔吸附树脂对獐牙菜苦苷的纯化效果较好,其动态饱和吸附量为70.48mg/g,依次用蒸馏水2BV、30%乙醇4BV洗脱,收集30%乙醇洗脱液,喷雾干燥,干粉样品中獐牙菜苦苷的含量为52.29%。结论HPD-100型大孔吸附树脂对獐牙菜苦苷纯化效果较好,适合于当药中环烯醚萜类成分獐牙菜苦苷的富集纯化,可用于生产。Objective To explore the suitable type of macroporous adsorbent resin to purity swertiaman from Swertia pesudochinensis Hara., and determine the parameters and conditions for its purification procedures. Method Purification effect of 8 types of macroporous resins used to purify the Swertia pesudochinensis Hara. were inspected, with the content of swertiaman as index. Result HPD-100 type resin showed better effect than others, it had optimum adsorption and elution parameters with its dynamic saturated adsorption ratio was 70.48 mg/g. After eluted with 2 BV of distilled water, 4 BV 30% ethanol and collected, the content of swertiaman in the spray dried powder was 52.29%. Conelusion HPD-100 types macroporous resin showed a better comprehensive adsorption property and can be used to enrich and purify the swertiaman included active fraction of iridoid glycosides for industrialization.

关 键 词：当药 环烯醚萜 獐牙菜苦苷 大孔吸附树脂 HPD-100 

分 类 号：R283.5[医药卫生—中药学]