青钱柳茎段离体培养研究  

In Vitro Culture of Stem Segments of Cyclocarya paliurus

作　　者：胡冬南[1] 上官新晨[2] 刘亮英[1] 谢风[1] 郭春兰[2] 

机构地区：[1]江西农业大学江西省竹子种质资源与利用重点实验室,南昌330045 [2]江西农业大学植物资源开发与利用研究室,南昌330045

出　　处：《湖北农业科学》2009年第6期1300-1303,共4页Hubei Agricultural Sciences

基　　金：教育部林木;花卉遗传育种重点实验室(北京林业大学)开放基金项目(HX2006-3-12);江西省教育厅科研项目(JXJY2004-3-69)

摘　　要：以青钱柳嫩枝茎段为外植体,研究了基本培养基、茎段部位、取样时间和植物生长调节剂对其分化的影响。当以青钱柳嫩枝茎段为外植体进行组织培养时,改良MS是适宜的基本培养基,5月份是最佳的取样时期,顶芽下1、2节的茎段分化效果较好;在培养基中添加0.5 mg.L-1反式玉米素(ZT)能有效地促进分化,萌发率最高能达到90.9%,平均每茎段分化的芽数达到1.5个。所以,茎段离体培养是青钱柳快速繁殖的有效途径。In this study, the effects of basic medium, sampling position, time for sampling explants and plant growth regulator on in vitro culture of stem segments of Cycloearya paliurus （Batal.） Iljinsk. were studied. The improved MS was better when the stem segments of shoot was cultivated in vitro. May was the best time for collecting explant. The first and the second nodes from the top bad a higher differentiation rate. 0.5 mg ·L^-1 ZT could accelerate the stem segment to flush, the highest differentiation rate was 90.9% and the mean of buds was 1.5 per stem segment. In vitro culture of stem segments was an effective method.

关 键 词：青钱柳 茎段 组织培养 

分 类 号：Q949.735[生物学—植物学] Q943.1