采用体外报告基因试验方法研究妊高征患者总雌激素效应  

Study on Serum Total Estrogen Activity of Patients with Pregnancy-induced Hypertension Syndrome by in Vitro Reporter Gene Assay

作　　者：曹新国[1] 孙宏[2] 王小青[1] 郭锡熔[1] 

机构地区：[1]南京医科大学附属南京妇幼保健院,江苏南京210004 [2]江苏省疾病预防控制中心环境卫生科

出　　处：《实用预防医学》2009年第4期1007-1009,共3页Practical Preventive Medicine

基　　金：南京医科大学科技发展基金(06NMUM052)

摘　　要：目的建立检测人血清总雌激素活性的报告基因试验方法,研究妊娠高血压综合征与血清环境雌激素水平的关系。方法采用ER介导的报告基因试验方法,检测了15例正常孕妇和10例妊高征患者的血清总雌激素活性。结果rERa报告基因系统能够很好的反映人血清中的总雌激素活性;妊高征患者血清总雌激素活性显著高于正常孕妇对照(P<0.01)。结论妊高征患者血清环境雌激素活性可能高于正常对照,这可能和妊高征的发生和发展密切相关。Objective To establish a reporter gene assay method in determination of human serum total estrogenic activity, and to study the relationship between pregnancy- induced hypertension syndrome and the level of environmental estrogens. Methods Serum total estrogen activity in 15 normal pregnant women and 10 patients with pregnancy- induced hypertension syndrome was examined by estrogen; receptors induced in vitro reporter gene assay. Results rERa reporter gene assay showed good effects on human serum total estrogenic activity. Serum total estrogenic activity in patients with pregnancy- induced hypertension syndrome were significantly higher than that in normal pregnant women.（P〈0.01）. Conclusions Serum environmental estrogenic activity in patients with pregnancy- induced hypertension syndrome may be higher than that of normal pregnant women, which may play an important role in the occurrence and development of pregnancy- induced hypertension.

关 键 词：环境雌激素 妊娠高血压综合征 报告基因实验 

分 类 号：R714[医药卫生—妇产科学]