河北省2008年脊髓灰质炎疫苗株病毒监测分析  

Monitoring of Polio Vaccine Virus Strains in Hebei Province in 2008

作　　者：陈玫[1] 郭玉[1] 张俊棉[1] 张振国[1] 赵娜[1] 崔志强[1] 

机构地区：[1]河北省疾病预防控制中心免疫规划管理所,河北石家庄050021

出　　处：《实用预防医学》2009年第4期1238-1239,共2页Practical Preventive Medicine

摘　　要：目的分析河北省脊髓灰质炎(脊灰)疫苗株病毒的监测情况,及时监测可能出现的脊髓灰质炎疫苗衍生病毒(VDPV)以防止其扩散。方法按世界卫生组织《脊髓灰质炎实验室手册》对河北省2008年急性弛缓性麻痹(AFP)病例及其密切接触者粪便标本进行分离与血清定型,用逆转录-聚合酶链反应(RT-PCR)法扩增目的基因片段,对扩增产物进行核苷酸序列测定和分析。结果从407例AFP病例粪便标本中,分离到脊灰疫苗株病毒24株,分离率为5.90%,从24例AFP密切接触者粪便标本中分离到2株脊灰疫苗株病毒。对分离到的39株单血清型PV进行了VP1基因核苷酸序列测定和分析,均未发现VDPV和脊灰野病毒。结论2008年河北省继续保持无脊灰状态,实验室监测系统敏感有效,为维持无脊灰状态提供了可靠的实验室依据。Objective To study the surveillance indicators in polio laboratory of Hebei Province, and to monitor vaccine - derived poliovirus （VDPV） or wild- type poliovirus in time. Methods According to WHO＇s standard method to isolate virus, then the neutralization test and intratypic differentiation test were carried out to all polioviruses isolated using PCR- RFLP methods. Results All the surveillance indicators in polio laboratory of Hebei met the requirements of WHO and MOH. 24 strains of polioviruses were isolated from 407 AFP cases, and the isolation rate was 5.90 %. The 39 strains of single serotype PVs were performed VP1 sequencing, and no VDPV or wild - type poliovirus was found. Conclusions Polio- free status was maintained in 2008 in Hebei Province. The laboratory surveillance system was sensitive and laboratory data were provided for maintaining the polio- free status in Hebei Province.

关 键 词：脊髓灰质炎病毒 急性弛缓性麻痹 脊髓灰质炎疫苗衍生株病毒 

分 类 号：R373.22[医药卫生—病原生物学]