鲑精蛋白对致病性弧菌的抑制活性  

Antibacterial Effect of the Salmine against Pathogenic Vibrio Species

作　　者：鲁健章[1,2] 苏意诚 刘承初[1] 

机构地区：[1]上海海洋大学食品学院,上海200090 [2]浙江省医学科学院保健食品研究所,杭州310013 [3]美国俄勒冈州立大学海洋食品研究与教育中心,俄勒冈97103

出　　处：《天然产物研究与开发》2009年第3期461-464,共4页Natural Product Research and Development

基　　金：上海市重点学科建设项目(编号:T1102);上海市属高校高水平特色项目-海洋生物资源与环境创新平台(编号:6870309);上海市教委科研创新项目(09ZZ166)

摘　　要：为了解鲑精蛋白对致病性弧菌——副溶血性弧菌(Vibrio parahaemolyticus BE-98-2029)、霍乱弧菌(Vibriocholerae CDC 2164)和创伤弧菌(Vibrio vulnificus ATCC27562)的抑菌活性,本文分别测定了2000～4000μg/mL浓度的鲑精蛋白对三种弧菌在TSB(大豆肉汤)+1.5%NaCl培养基中的生长抑制情况和对菌体的致死时间。试验结果表明,2000μg/mL鲑精蛋白可有效抑制三种弧菌的生长,使霍乱弧菌48h内完全死亡,使副溶血性弧菌数量从3.3×106cfu/mL降至3.3×103cfu/mL;3000μg/mL的鲑精蛋白则可以在48h内完全杀死三种弧菌;4000μg/mL的鲑精蛋白在12h内使创伤弧菌总数从4.9×106cfu/mL降至9.4×102cfu/mL。The antibacterial activities of salmine against three pathogenic Vibrio species （ Vibrio cholerae CDC 2164, Vibrio parahaemolyticus BE-98-2029 and Vibrio vulnificus ATCC 27562） were studied at concentrations ranging from 2000 to 4000 μg/mL. Growth of Vibrio cholerae CDC 2164 was inhibited by salmine at a concentration of 2000 μg/mL and its populations were reduced to non-detectable levels in 48 h. The same concentration （2000 μg/mL） also reduced populations of Vibrio parahaemolyticus BE-98-2029 from 3.3 × 10^6 cfu/mL to 3.3 × 10^3 cfu/mL after 48 h. Increasing the concentration of salmine to 3000 μg/mL was able to reduce the populations of Vibrio parahaemolyticus BE-98-2029 to nondetectable levels in 48 h. A higher concentration of salmine （4000 μg/mL） was capable of reducing the populations of Vibrio vulnificus ATCC 27562 from 4.9 × 10^6 to 9.4 × 10^2 cfu/mL in 12 h.

关 键 词：鲑精蛋白 副溶血弧菌 霍乱弧菌 创伤弧菌 抑菌效果 

分 类 号：Q512.8[生物学—生物化学] R965[医药卫生—药理学]