大孔吸附树脂提取7-木糖-10-去乙酰紫杉醇酶解产物10-去乙酰紫杉醇  

Extraction of 10-Deacetylpaclitaxel,the Enzymic Hydrolysis Products of 7-Xylosyl-10-Deacetylpaclitaxel,by Macroporous Adsorption Resin

作　　者：刘兴宝[1] 栾宏伟[1] 葛广波[1,2] 闫黎[1] 杨凌[1] 

机构地区：[1]中国科学院大连化学物理研究所,大连116023 [2]中国科学院研究生院,北京100039

出　　处：《天然产物研究与开发》2009年第3期496-501,共6页Natural Product Research and Development

基　　金：中国科学院知识创新基金(K2006A9);大连市科技计划项目基金(2007E11SF0052)

摘　　要：本研究利用大孔树脂富集7-木糖10-去乙酰紫杉醇的酶解产物10-去乙酰紫杉醇。首先以10-去乙酰紫杉醇(10-DAT)绝对含量为指标,通过HPLC测定比较HP20、XAD4、XAD16、XAD1600四种大孔树脂对10-去乙酰紫杉醇的吸附与解吸性能力,筛选出最佳树脂XAD1600进行实验。最佳的吸附解吸条件为:树脂柱高径比10∶1,吸附流速20mL/min,吸附量23.85mg/mL,以6倍柱床体积的90%乙醇为洗脱液,洗脱流速20mL/min。经XAD1600型树脂富集后10-去乙酰紫杉醇回收率达96.5%,含量20.5%。且树脂柱重复使用3次后,其吸附能力仅降低5%。In order to obtain 10-deacetylpaclitaxel, the products of enzymic hydrolysis of 7-xylosyl-10-deacetylpaclitaxel, four candidate macroporous resins （ HP20, XAD4, XAD16, XAD1600 ） were compared. The results showed that the macroporous resin XAD1600 had the highest dynamic adsorption capacity. Therefore, it was selected to further study on the optimized eonditions for adsorption and desorption of 10-deacetylpaelitaxel. The optimal conditions was as follows：the ratio of height-diameter （10：1 ） ,the dynamic adsorption capacity （23.85 mg/mL）, the adsorption flow rate （20 mL/ rain） ,elution solvent （90% ethanol,6 BV） and the elution flow rate （20 mL/min）. The recovery and content of 10- deacetylpaclitaxel by this technological process was up to 96.5% and 20.5% respectively. Additionally, XAD1600 macroporous resin presented a good stabilization, and the adsorption rate only decreased 5% after using three times.

关 键 词：7-木糖-10-去乙酰紫杉醇 10-去乙酰紫杉醇 大孔树脂 吸附与解吸 

分 类 号：R979.9[医药卫生—药品] Q946.91[医药卫生—药学]