缬草抗心律失常有效部位的HPLC指纹图谱研究  

HPLC Fingerprint of the Antiarrhythmic Fraction of Valeriana officinalis

作　　者：段雪云[1,2] 龚占峰[1] 陈树和[2] 方颖[1] 刘焱文[1] 

机构地区：[1]湖北中医学院湖北省中药资源与中药复方省部共建教育部重点实验室,湖北武汉430061 [2]湖北省中医院,湖北武汉430061

出　　处：《中药材》2009年第6期866-870,共5页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金项目(30500053)

摘　　要：目的:建立缬草抗心律失常有效部位的指纹图谱。方法:采用高效液相色谱法。色谱柱为Agilent C18色谱柱(250mm×4.6mm,5μm);流动相为乙腈-水溶液,梯度洗脱;检测波长:218nm;柱温:38℃;流速:1.0mL/min。结果:建立了缬草抗心律失常有效部位的HPLC-UV指纹图谱,标定了15个共有指纹峰,确定了其相对保留时间和相对峰面积的范围。结论:该方法准确、可靠,可为缬草的质量控制提供参考。Objective： To establish HPLC fingerprints of the Antiarrhythmic fraction of Valeriana officinalis. Methods： Agilent C18 （250 mm× 4. 6 mm,5 μm） column was used and the acetonitrile-water was chosen as the mobile phase in a gradient mode. The column temperature was 380℃ and the detection wavelength was 218 nm. The detection time was 70 rain,and the flow rate was 1.0 mL/ min. Results ： Fifteen characteristic peaks were indicated in HPLC fingerprints. The relative retention time and the ranges of relative are- as of the common peaks were also determined. Conclusion ： This method is simple and accurate with a good reproducibility and provides a reference standard for the quality control of Valeriana officinalis.

关 键 词：缬草 指纹图谱 抗心律失常 

分 类 号：R282.5[医药卫生—中药学]