金钗石斛中黄酮类和酚类成分HPLC指纹图谱研究  

HPLC Fingerprint of Flavonoids and Phenols of Dendrobium nobile

作　　者：欧焕娇[1,2] 成金乐 李先霞[3] 詹若挺[1] 梁逸曾[4] 徐吉银 徐晖[1] 严萍[1] 

机构地区：[1]广州中医药大学中药资源科学与工程研究中心,广东广州510006 [2]中山市中智药业集团有限公司,广东中山528437 [3]湖南岳阳职业技术学院,湖南岳阳414000 [4]中南大学,湖南长沙410083

出　　处：《中药材》2009年第6期871-874,共4页Journal of Chinese Medicinal Materials

基　　金：中山市科技计划(2006A084);广东省高等学校学科建设专项(粤教科函[2008]3号)

摘　　要：目的:建立金钗石斛药材中黄酮类和酚类成分的HPLC指纹图谱。方法:采用Phenomenex prodigy ODS(3)C18色谱柱(250mm×4.6mm,5μm),乙腈-0.1%乙酸溶液梯度洗脱,柱温25℃,流速1.0mL/min,检测波长254nm。结果:该方法可使金钗石斛中黄酮类和酚类成分得到较好的分离,并根据检测结果选定了10个共有指纹峰。结论:该法操作简便、重现性好,可作为金钗石斛药材质量控制的方法。Objective ： To establish HPLC fingerprint of flavonoids and phenols of Dendrobium nobile. Methods ： Phenomenex prodigy ODS（ 3 ）Cls column （250mm×4.6mm,5μm）was used with a mixture of aeetonitfile-0. 1% acetic acid as the mobile phase in a gradient mode, the column temperature was 25℃, the flow rate was 1.0 mL/min, and the detection wavelength was 254 tun. Resuhs：The flavonoids and phenols of Dendrobium nobile were well separated, and 10 fingerprint peaks in common were confirmed. Conclusion： This method is simple, accurate with good reproducibility, and can be used specifically for the quality control of Dendrobium nobile.

关 键 词：金钗石斛 黄酮类和酚类 指纹图谱 高效液相色谱 

分 类 号：R282.5[医药卫生—中药学]