柱前衍生HPLC法分析白土茯苓多糖中单糖的组成  

Analysis of Monosaccharide Compositions in Heterosmilax japonica Polysaccharide by Precolumn Derivation HPLC

作　　者：韩晶[1] 陈晓辉[1] 孙立新[1] 占志惠[1] 毕开顺[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《中药材》2009年第6期893-895,共3页Journal of Chinese Medicinal Materials

摘　　要：目的:建立柱前衍生HPLC法测定白土茯苓多糖中单糖的组成。方法:以三氟乙酸(TFA)水解白土茯苓多糖,水解产物加入1-苯基-3-甲基-5-吡唑啉酮(PMP)进行衍生化,采用反相高效液相色谱法,测定白土茯苓多糖中单糖的衍生物。色谱柱:KromasilC18柱(250mm×4.6mm,5μm);流动相:100mmol/L醋酸铵缓冲液(pH=5.5)∶乙腈=77∶23(V/V);检测波长:245nm。结果:白土茯苓多糖中含有甘露糖、鼠李糖、葡萄糖、半乳糖和木糖,其摩尔比为66∶1∶7590∶452∶528。结论:该方法简便、准确,可以测定出白土茯苓多糖中单糖的组成。Objective：To establish precolumn derivation HPLC method to determine the monosaccharides in Heterosmilax japonica polysaccharide. Methods：Heterosmilax japonica sample was hydrolyzed with trifluoroacetic（TFA） and derivated by 1-phenyl-3methyl-5- pyrazolone（PMP）. The monosaccharide composition of Heterosmilax japonica polysaccharide was carried out by reversed-phase technique on a Kromasil C18 column with a mobile phase composed of 100 mmol/L ammonium acetate buffer（ pH5.5 ）and acetonitrile in the ratio of 77： 23. The detection was carried out at 245 nm. Results：The Heterosmilax japonica polysaccharide composed of mannose, rhamnose, glucose, galactose and xylose with amolar ratio of 66：1 ： 7590： 452： 528. Conclusion： The HPLC method with precolumn derivatization is appropriate for the analysis of monosaccharide composition of Heterosmilax japonica polysaccharide and the method is simple, quick and accurate.

关 键 词：白土茯苓多糖 柱前衍生化 HPLC 单糖 

分 类 号：R284.1[医药卫生—中药学]