夏枯草提取物作用Jurkat细胞的蛋白质组学研究  

Study on Proteomics of Jurkat Cells Treated with the Extracts from Prunella vulgaris

作　　者：张明智[1] 孙振昌[1] 付晓瑞[1] 陈长英[1] 丁梦杰[1] 

机构地区：[1]郑州大学第一附属医院肿瘤科,河南郑州450052

出　　处：《中药材》2009年第6期917-922,共6页Journal of Chinese Medicinal Materials

摘　　要：目的:研究夏枯草提取物作用于Jurkat细胞后蛋白质组的变化。方法:体外培养Jurkat细胞,用MTT法观察不同浓度夏枯草提取物对细胞的增殖抑制作用。加入20μg/mL夏枯草提取物作用于细胞48 h,提取总蛋白,进行双向电泳测定,凝胶银染显色,用ImageMaster 2D Platium 5.0软件对获得的蛋白图谱加以分析,寻找差异表达的蛋白质。切取差异点,胶内酶切后进行MALDI-TOF-MS分析和数据库搜索,实现对蛋白点的定性鉴定。结果:夏枯草提取物可显著抑制Jurkat细胞的生长,且具有一定的量效关系。经双向电泳和质谱后,成功鉴定了11个蛋白质,包括glyceraldehyde-3-phosphate dehydrogenase、coagulation factor VII、Heterogeneous nuclear ribonucleoprotein L、heat shock 70 kDa protein 8 isoform 2、immunoglobulin heavy chain variable region、heterogeneous nuclear ribonucleoprotein A2/B1、heterogeneous nuclear ribonucleoprotein L(为heterogeneous nuclear ribonucleoprotein A2/B1不同亚型)、zinc finger protein 43、chaperonin containing TCP1、subunit 6A (zeta 1)、isoform CRA-b。结论:夏枯草提取物可显著抑制Jurkat细胞的生长,并引起Jurkat细胞蛋白质组的改变,这可能是夏枯草提取物抗肿瘤作用的机制之一。Objective ： To analyse the differential proteins of Jurkat cells after treated with the extracts from Prunella vulgaris using two-dimensional electrophoresis and mass spectrum. Methods：Jurkat cell growth inhibitive effect of the extracts from Prunella vulgaris was analyzed by MTT assay. The total proteins of the cells were extracted after treated with the extracts in a dose of 20μg,/mL Then 2D and MALDI-TOF-MS were used to assess the differential proteins. Results：The extracts from Prunella vulgaris could depress the proliferation of Jurkat cells in a dose-dependent manner. After 2-DE and MALDI-TOF-MS, 11 proteins were identified successfully, including glyceraldehyde-3-phosphate dehydrogenase, coagulation factor VII, Heterogeneous nuclear ribonucleoprotein L, heat shock 70 kDa protein 8 isoform 2,immunoglobulin heavy chain variable region, heterogeneous nuclear ribonucleoprotein A2/B1, zinc finger protein 43, chaperonin containing TCP1, subunit 6A （zeta 1 ） , isoform CRA_b, etc. Conclusion：The extracts from Prunella vulgaris could inhibit the growth of Jurkat cells significantly, and lead the proteomics change of Jurkat cells, which may be related to the anti-tumor effect of the extracts from Prunella vulgaris.

关 键 词：夏枯草 JURKAT细胞 蛋白质组学 MALDI—TOF—MS 

分 类 号：R285.5[医药卫生—中药学]