雄黄微生物浸出液对S_(180)腹水瘤的生长抑制效应及其毒性研究  

The Inhibitory Effects of Realgar Bioleaching Solution on S_(180) Ascites-tumor and Its Toxicity

作　　者：谢亲建[1] 张旭[2] 王欣[2] 白璐[2] 李红玉[1] 

机构地区：[1]兰州大学药学院,甘肃兰州730000 [2]兰州大学生命科学学院干旱与草地生态教育部重点实验室,甘肃兰州730000

出　　处：《中药材》2009年第6期933-936,共4页Journal of Chinese Medicinal Materials

基　　金：教育部重点科技项目(107108);甘肃省科技攻关项目(2GS035-A52-008-01;2GS064-A43-019-02);甘肃省科技计划资助(0708WAGA150)

摘　　要：目的:研究雄黄微生物浸出液中无机砷抑制肿瘤S180的作用,并评价其毒性,为雄黄的二次开发提供参考依据。方法:建立小鼠S180腹水瘤模型,24h后给药,1次/天,然后观察15d的生命延长率;按照改良寇氏综合计算法,分析3种不同的给药方法(灌胃、腹腔、静脉)对小鼠的急性毒性。结果:矿物药雄黄浸出液中无机砷抑制肿瘤S180瘤株有明显的作用,并呈剂量依赖性,对S180腹水瘤的15d生存率达到10%～60%;3种给药方法的LD50值分别为5.27、3.63、2.68mg/kg。结论:矿物药雄黄浸出液中无机砷具有抗肿瘤作用,其效果强于三氧化二砷注射液,但毒性强于三氧化二砷注射液。Objective： To investigate the inhibitory effect of Realgar bioleaching solution（RBS） on tumor S180 cells line and estimate its toxicity, to provide experimental evidence for the further exploit of Realgar. Methods：24 hours after the models of ascites-tumor bearing mice were established, the mice were injected RBS once a day. The survival rates of S180 ascites-tumor bearing mice injected in RBS was studied, and the RBS acute toxicity of mice produced by oral, intraperitoneal or intravenous was evaluated by Drug Median Lethal Dose （ LD50 ）. Results ： The inhibitory effect of S180 cells in vivo had a dose-dependent manner. The survival rates of mice were 10% -60% in the different dose in 15 days. The LDso values of RBS by oral, intraperitoneal or intravenous were 5.27 mg/kg, 3.63 mg/kg and 2.68 mg/kg, respectively. Conclusion ： RBS has potent antitumor effect, LD50 value of RBS is lower than that of traditional medicine.

关 键 词：雄黄 腹水瘤 肿瘤S180 毒性 LD50 

分 类 号：R285.5[医药卫生—中药学]