抗残杀威和敏感家蝇乙酰胆碱酯酶生化特性研究  

Study on Biochemical Properties of Acetylcholinesterase from Propoxur Resistant and Susceptible Strains of the Housefly,Musca domestica

作　　者：袁建忠[1,2] 孙晓琴[1] 唐振华[1,2] 陶黎明[1,3] 

机构地区：[1]华东理工大学药学院,上海200237 [2]中国科学院上海生命科学院上海植物生理生态研究所,上海200032 [3]上海市农药研究所,上海200032

出　　处：《农药学学报》2009年第2期181-185,共5页Chinese Journal of Pesticide Science

基　　金：国家重点基础研究发展规划(“973”计划)项目(2003CB114403)

摘　　要：对家蝇残杀威抗性(RR)和敏感(SS)品系乙酰胆碱酯酶(acetylcholinesterase,AChE)(RR-和SS-AChE)的生化特性研究后发现,RR-和SS-AChE存在着明显的差异:1)RR-和SS-AChE最适反应温度分别为37℃和34℃,但最适pH值均为7.4;2)AChE催化底物的能力不同,RR-AChE水解碘化硫代乙酰胆碱(ATCh)、碘化硫代丁酰胆碱(BTCh)、碘化硫代丙酰胆碱(PTCh)的活力高于SS-AChE,其相应的最大反应速率(Vmax)比分别是SS-AChE的2.22、1.08和3.41倍;3)从双分子速率常数(bimolecular constant,Ki)来看,RR-AChE对4种氨基甲酸酯类抑制剂(残杀威、克百威、甲萘威、灭多威)的敏感度分别是SS-AChE的46.77、28.15、66.15和15.00倍,对4种有机磷类抑制剂(马拉氧磷、甲胺磷、氧乐果和氧化三唑磷)的敏感度分别是7.66、12.13、3.81和2.25倍;4)上述抑制剂与RR-AChE分子相互作用的亲和力常数(Ka)均大于与SS-AChE的值;5)RR-AChE的磷酰化或氨基甲酰化常数(K2)值都低于SS-AChE的值。表明RR-AChE的性质已发生变化。It was found by study of biochemical properties of acetylcholinesterase （ RR-and SS-AChE） that the propoxur-resistant （RR） and susceptible （SS）-AChe of the housefly, Musca domestica were different in the following aspects. 1 ） The optimum temperature of AChE in the RR and SS strains was found at 37~C and 34~C, respectively. But the optimum pH value was all the same, 7.4. 2 ） The capability of substrate hydrolyzed by the two AChEs existed markedly difference. The Vmax ratios of RR- to SS-AChE were 2.22 for ATCh, 1.08 for BTCh and 3.41 for PTCh. 3 ） Based on the bimolecular rate constant （Ki）, the RR-AChE was 46.77-,28. 15-,66. 15- and 15.00-fold less sensitive to four carbamatespropoxur, carbofuran, carbaryl and methomyl and was 7.66 -, 12.13-, 3.81 and 2.25 - fold less sensitive to four organophosphates malaoxon, methamidophos, omethoate and triazophos oxon, respectively. 4 ）The affinity constant （Ka ） values of the tested inhibitors with RR-AChE were all higher than that of SS-AChE. 5 ） The phosphorylation or carbamylation constant （ K2 ） values of RR- AChE were all less than that of SS-AChE. All these data implied that RR-AChE was qualitatively altered.

关 键 词：家蝇 杀虫剂 乙酰胆碱酯酶 抗药性 酶动力学分析 

分 类 号：S481.4[农业科学—农药学] Q965.9[农业科学—植物保护]