麦田抗性生物型猪殃殃对苯磺隆的抗性机制  

Mechanism of Resistance to Tribenuron-methyl in a Resistant Biotype of Galium aparine

作　　者：彭学岗[1] 王金信[1] 刘君良[1] 杨纪辉[1] 

机构地区：[1]山东农业大学植物保护学院,山东泰安271018

出　　处：《农药学学报》2009年第2期191-196,共6页Chinese Journal of Pesticide Science

基　　金："十一五"国家科技支撑计划(2006BAD08A09)

摘　　要：为探讨猪殃殃Galium aparine抗药性生物型(R)对苯磺隆的抗性机制,测定了苯磺隆对猪殃殃抗性、敏感(S)生物型体内靶标酶[乙酰乳酸合成酶(ALS)]、代谢酶[谷胱甘肽-S-转移酶(GSTs)]及抗氧化酶[超氧化物歧化酶(SOD)、过氧化物酶(POD)]影响的差异。离体试验结果表明,苯磺隆对R、S生物型猪殃殃ALS的抑制中量(IC50)分别为0.682、0.718μg/L(有效剂量),R、S生物型猪殃殃ALS对苯磺隆的敏感性不存在差异。活体试验结果表明,苯磺隆茎叶喷雾处理后,R、S生物型ALS活力均表现为先上升,但S生物型上升幅度小,且随后快速下降,第3d即回落至对照之下,并维持在低于对照的水平,而R生物型ALS活力在第2d可达对照的4.10倍,第5d基本回落至对照水平,之后基本维持在对照水平;R生物型GSTs活力在第1d即开始上升,最高可达对照的2.40倍,而S生物型则表现为先下降,然后小幅回升,最高为对照的1.61倍,两者在10d左右均回落至对照水平;R生物型SOD活力与对照基本相同,而S生物型虽略有下降,但R、S间不存在显著差异;两者POD活力虽均有大幅提高,但亦不存在显著差异。结果表明,低水平抗药性生物型猪殃殃对苯磺隆产生抗性的原因可能是ALS过量表达及GSTs对苯磺隆的代谢作用加强,而不是由于ALS的敏感性下降,同时POD、SOD在减轻药害中也具有一定作用。In order to study the mechanism of the biotype of Galium aparine resistant to tribenuron- methyl,the differences on ALS, GSTs, SOD and POD of the resistant （R） and susceptible （S） biotypes to tribenuron-methyl were preliminarily determined. There was no significant difference between the sensitivity of ALS in vitro from the R and S biotypes to tribenuron-methyl, with the IC50, 0. 682 and 0. 718 ixg （a. i. ）/L, respectively. The ALS activity in vivo of both R and S biotypes increased at the first 2 days after application of tribenuron-methyl, however, the ALS activity in vivo of S biotype increased much less and declined much more quickly,less than the control value 3 days after treatment and lasted out,while that of R biotype is 4.10 times of the control,and declined slowly to the control value 5 days later and kept on. Meanwhile, the GSTs activity of R biotype increased immediately, with the peak value 2.40 times of the contemporary control, while that of S biotype declined at first and then increased,with peak value 1.61 times. Both of them came down to the control value 10 days later. The SOD activity of R biotype maintained the control level, while that of S biotype declined a little, but no significant difference existed. The POD activity of both biotypes increased greatly, but no significant difference existed either. The results indicate that the low level resistance to tribenuron-methyl in the biotype of Galium aparine from Xuchang, Henan Province, is based on the excessive expression of ALS and the enhancement of tribenuron-methyl metabolism resulting from GSTs, not due to the sensitivity of ALS. In the meanwhile POD and SOD may play a role in alleviating the spray injury.

关 键 词：猪殃殃 苯磺隆 杂草 抗药性 机制 

分 类 号：S481.4[农业科学—农药学]