柴胡、红花、川芎中药单体对大鼠肝纤维化治疗作用的实验研究  

Experimental study on inhibiting effects of bupleurum chinense,carthamus tinctorius and szechwan lovage rhizome on rat with hepatic fibrosis

作　　者：杨洁[1] 蔡刁龙 谭献文[3] 吕永慧[1] 田立新[1] 康宜宾[1] 余幼鸣[1] 

机构地区：[1]广州市中医医院消化内科,广东广州510130 [2]陆丰市甲子人民医院外科,广东陆丰516538 [3]广州市第一人民医院药剂科,广东广州510180

出　　处：《临床和实验医学杂志》2009年第7期1-3,共3页Journal of Clinical and Experimental Medicine

基　　金：广东省科技计划项目(2008B060600012);广东省中医药局建设中医药强省科研课题(2008017);广州市中医药和中西医结合科研课题项目(2008A14)

摘　　要：目的比较柴胡、红花、川芎3种中药单体抗大鼠肝纤维化的疗效并探讨其作用机制。方法以生理盐水为对照,将3种中药用于大鼠肝纤维化模型和大鼠肝星状细胞(HSC)。观察肝组织形态变化;检测血透明质酸酶、Ⅳ型胶原含量;MTT法检测HSC细胞增殖抑制率;免疫组化法检测基质金属蛋白酶-1(MMP-1)、基质金属蛋白酶组织抑制因子-1(TIMP-1),转化生长因子β1(TGFβ1),血小板衍生生长因子(PDGF)的表达。结果与对照组比较,中药处理组的HSC细胞增殖抑制率升高(P<0.05);血清透明质酸、Ⅳ型胶原含量减少(P<0.05);肝组织TIMP-1、TGFβ1及PDGF的表达均减少(P<0.05)。结论3种中药均能减缓实验性肝纤维化的发生。其机制可能是这些中药能下调TGFβ1及PDGF表达抑制HSC增殖活化,从而下调TIMP-1/MMP-1而促进细胞外基质降解,减少其沉积。Objective To study the anti - fibrotic effect of bupleurum chinense, carthamus tinctorius and szechwan lovage rhizome and their possible mechanisms. Methods The three Chinese traditional drugs were given to rats with hepatic fibrosis and hepatic stellate cells （HSC） culture in vitro. Natural sodium was administered or added as the control. The following indexes were examined including the degree of hepatic fibrosis, serum levels of hyaluronic acid （HA） and type IV collagen （ C Ⅳ ） , expressions of tissue inhibitors of matrix metalloproteinase - 1 （ TIMP - 1 ） , matrix metalloproteinase - 1 （ MMP - 1 ） , ransfonning growth factor β1 （ TGFβ1 ） and platelet - derived growth factor （PDGF） in liver tissues. HSC proliferation was evaluated by MTF assay. Results Compared with the control group, the serum level of HA and CⅣ decreased significantly ; expressions of TIMP - 1, TGFβ1 and PDGF decreased significantly in all drug - treated groups （ P 〈 0.05 ）. Conclusion Bupleurum chinense, carthamus tinctorius and szechwan lovage rhizome inhibit hepatic fibrosis. They may play roles through decreasing TGFβ1 and PDGF expressions and the ratio of TIMP - 1 to MMP - 1 to inhibit HSC activation and proliferation to decrease extracellular matrix.

关 键 词：肝纤维化 肝星形细胞 中药单体 柴胡 红花 川芎 动物实验 

分 类 号：R285[医药卫生—中药学]