同时测定血府逐瘀汤中3种主要成分的含量  

Simultaneous Determination Content of Three Main Components in Xuefu Zhuyu Decoction by HPLC

作　　者：于红宇 李艳卿 陈历雄 张军[2] 林雪颖[2] 陈汀波[2] 李文周[2] 

机构地区：[1]广州番禺何贤纪念医院,广东广州511400 [2]广州中医药大学新药开发研究中心,广东广州510405

出　　处：《今日药学》2009年第6期36-38,63,共4页Pharmacy Today

摘　　要：目的建立以高效液相色谱法(HPLC)同时对血府逐瘀汤中羟基红花黄色素A、芍药苷和柚皮苷等3种主要成分进行含量测定的方法。方法采用Kromasil C18(250mm×4.6mm,5μm)色谱柱;以甲醇-乙腈(26∶2)-0.7%磷酸为流动相,进行梯度洗脱;检测波长为403、283、230nm;流速为1ml/min。结果3种成分分离效果良好,羟基红花黄色素A、芍药苷、柚皮苷加样回收率分别为101.1%(相对标准偏差值RSD=2.1%)、99.9%(RSD=1.1%)、102.1%(RSD=1.3%)。结论本法简单、快捷,适用于测定血府逐瘀汤中羟基红花黄色素A、芍药苷和柚皮苷的含量。Objective To develop a HPLC method for the determination of hydroxy safflower yillow A, paeoniforin, and aringin in the Xuefu Zhuyu decoction . Methods Chromatographic separation was performed on a Kromasil C18 column （250 mm × 4.6 mm, 5 μm） using a linear gradient elution with methanol - acetonitrile （26：2） -0. 7 % phosphoric acid as mobile phase at a flow rate of 1.00 ml/min. The detection wavelength was set at 230,283, and 403 nm. Result Hydroxy safflower yellow A, paeoniflorin and naringin were well separated and the average recoverywas 101. 1 % （RSD = 2. 1% ）, 99.9% （RSD = 1.1% ）, and 102.1% （RSD = 1.3% ）, respectively. Conclusion The method is simple, rapid and suitable for the determination of 3 main components in the decoction for removing blood stasis in the chest.

关 键 词：血府逐瘀汤 羟基红花黄色素A 芍药苷 柚皮苷 HPLC 

分 类 号：R284.2[医药卫生—中药学]