云芝漆酶的生物降解与染料脱色作用研究  

Research on the Biodegradation and Dye Decolourization for Laccase Produced from Trametes versicolor

作　　者：姚朝阳[1] 郭伟云[2] 牛敬媛[1] 邵强[3] 李宗义[3] 

机构地区：[1]新乡医学院基础医学院,河南新乡453003 [2]新乡医学院生命科学技术系,河南新乡453003 [3]河南师范大学生命科学学院,河南新乡453007

出　　处：《安徽农业科学》2009年第20期9363-9365,9408,共4页Journal of Anhui Agricultural Sciences

基　　金：河南省科技攻关项目(001200217)

摘　　要：［目的］研究云芝漆酶的生物降解和染料脱色作用。［方法］以云芝HS03发酵后所得漆酶粗制品，对麦草和锯末进行生物降解，并对几种常用染料进行脱色研究。［结果］结果表明：漆酶粗制品与纤维素酶、木聚糖酶协同作用大大提高了木质纤维素降解率，比单一组分酶获得还原性糖含量提高5～6倍。漆酶粗制品具有广谱的脱色作用，尤其对孔雀石绿、RB亮蓝的脱色率都超过了85％，脱色较为彻底，脱色高峰期为48～72 h。[ Objective ] This research aimed to study the biodegradation and dye decolourization for laccase produced from Trametes versicolor. [ Method ] Crude Iaccase from T. versicolor HS-03 was used for the biodegradation of wheat straw and sawdust,and the decolourization of different molecular structure type＇s dye. [ Result ] It was indicated that the crude laccase raised the lignocellulose biodegradation efficiency which was about 5 - 6 doubles. Crude enzyme has wide spectrum decoloured function, especially the decoloring rate of malachite green, RB bright blue was over 85%. The rush hour of deeolourization was 48 -72 h. [ Conclusion]This study could provide theoretical basis for dye decolourization of waste water,and provide strong references for the application of laccase in water treatment industry industrialization.

关 键 词：云芝 漆酶 生物降解 染料 脱色 

分 类 号：Q93[生物学—微生物学]