惠楼山药的组织培养与快繁技术研究  

Tissue Culture and Rapid Propagation Technology of Huilou Yam

作　　者：周玉玲[1] 余慧琳[2] 孙凤岭[1] 姜曙光[1] 李鑫宇[1] 刘广卿[1] 张福娟[1] 

机构地区：[1]河南省商丘市农林科学研究所,河南商丘476000 [2]商丘职业技术学院,河南商丘476000

出　　处：《安徽农业科学》2009年第20期9407-9408,共2页Journal of Anhui Agricultural Sciences

摘　　要：［目的］研究惠楼山药组织培养与快繁技术。[方法]以惠楼山药茎尖部分作为外植体，以MS为基本培养基，添加不同浓度的6-BA、NAA、IAA、2,4-D、IBA，配制不同的培养基，对惠楼山药试管苗进行分化与诱导、继代与增殖及生根培养试验，并对试管苗进行炼苗与移栽，还提出脱毒快繁后的惠楼山药的栽培要点。［结果］ 经分化与诱导培养后的无毒茎尖分化苗接种到继代与增殖培养基中，5d后有愈伤组织形成，28～35d苗高3～5cm，将生长势强、健壮的苗转入生根培养基中，25d左右生根率达95%以上，生根28～35d的幼苗经炼苗和移栽后，成活率在90%以上。［结论］获得了惠楼山药茎尖培养脱毒与诱导、增殖和生根的培养基，以及生长所需要的外部环境条件和配套技术。[ Objective ] The study aimed to study the virus-free tissue culture and the rapid propagation technology. [ Method ] With yam shoot tip as explants, MS was used as the basic medium with different concn, of 6-BA, NAA ,IAA ,2,4-D and IBA to configure different media,the tests of differentiation and induction, subculture and proliferation as well as rooting culture on Huilou yam were carried out and the test-tube plantlets were hardened and transplanted. The key cultivation techniques for Huilou yam after virus-free rapid propagation were also put forward. [ Result] When non-toxic differentiated seedlings of shoot tip after differentiation and induction culture were inoculated on subculture and proliferation medium, callus formed after 5 d,and seedling height was 3 -5 cm after 28 -35 d. The strong seedlings were transferred into the rooting medium,and then they got the rooting rate of over 95% after about 25 d. Finally the seedlings that rooted for 28 -25 d had survival rate of over 90% after hardening and transplanting. [ Conclusion ] In the experiment, the media of detoxiflcation and induction, proliferation and rooting were obtained as well as the external environmental conditions for the plant growth and the matching technology.

关 键 词：惠楼山药 组织培养 快繁 培养基 

分 类 号：S603.6[农业科学—园艺学]