活血潜阳方对SHR心肌间质重构及AngⅡ、AT1、AT2的影响  

Effect of Prescription for Activating Blood Circulation and Checking Exuberance of Yang on SHR Myocardial Interstitium Reconstruction,Ang II,AT1 and AT2

作　　者：杨秀青[1] 符德玉[1] 青淑云[1] 钱岳晟[2] 周端[3] 

机构地区：[1]上海中医药大学附属岳阳中西医结合医院,上海200437 [2]上海市瑞金医院高血压研究所,上海200025 [3]上海中医药大学附属龙华医院,上海200032

出　　处：《南京中医药大学学报》2009年第4期271-274,F0003,共5页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：国家自然科学基金(30572381)

摘　　要：目的探讨活血潜阳方对自发性高血压大鼠(SHR)心肌间质重构的影响及其可能机制。方法10周龄雄性SHR随机分为4组:SHR对照组、中药组、西药组、中西合用组(活血潜阳方+缬沙坦),同龄WKY大鼠为正常对照组。于治疗7周及14周后酶免法及放免法测血清及心肌组织血管紧张素Ⅱ(AngⅡ)含量,FQ-PCR法测心肌AT1、AT2mRNA表达,光镜观察心肌细胞及间质胶原变化和胶原容积分数(CVF),并进行各项指标间的相关分析。结果与WKY大鼠比较,SHR血清及心肌AngⅡ含量显著增高、心肌AT1、AT2mRNA表达显著上调、CVF显著增加(P<0.01)。活血潜阳方治疗7周即可显著降低组织AngⅡ含量、下调AT1mRNA表达、减少CVF(P<0.01);治疗14周可使血清AngⅡ含量降低(P<0.05)。结论活血潜阳方可改善SHR心肌间质重构,其机理可能与降低循环及心肌组织AngⅡ含量、下调心肌组织AT1mRNA表达有关。OBJECTIVE To explore into the effect of the prescription for activating blood circulation and checking exuberance of yang on myocardial interstitium reconstruction of spontaneous hypertension rats （SHR, China） and the possible mechanism. METHOD Ten-week-old male SHR were randomly divided into 4 groups： SHR control group, Chinese medicine group, western medicine group and Chinese and western medicine group. A normal group consisted of WKY rats of the same age. Enzyme immunoassay and radio immunoassay were used seven and fourteen weeks after the treatment to determine the various indexes. RESULT Compared with WKY rats, SHR serum and myocardium Ang Ⅱ increased markedly in content, myocardium AT1 and AT2 mRNA expression rose significantly and CVF increased greatly （ P 〈 0.01 ）. The prescription for activating blood circulation and checking exuberance of yang could markedly decrease tissue Ang Ⅱ content and reduce AT1 mRNA expression and CVF seven weeks after the treatment （ P 〈 0.01）, and could reduce serum Ang Ⅱ content fourteen weeks after the treatment （ P 〈 0.05）. CONCLUSION The prescription for activating blood circu- lation and checking exuberance of yang can improve SHR myocardial interstitium reconstruction, the mechanism of which is probably related to its effect in reducing Ang Ⅱ content in the circulation and myocardial tissue and AT1 mRNA expression.

关 键 词：活血潜阳方 自发性高血压大鼠(SHR) 心肌间质重构 AngⅡ AT1 AT2 

分 类 号：R285.5[医药卫生—中药学]