CCK-8法和MTT法检测人前列腺癌PC3细胞活性的最佳条件比较研究  

Optimal Experiment Conditions of Comparison between CCK-8 and MTT in Cell Proliferation Assays on PC3 Cell

作　　者：李宁[1] 万文婷[1] 金林红[1] 

机构地区：[1]贵州大学精细化工研究开发中心教育部绿色农药与生物工程重点实验室,贵州贵阳550025

出　　处：《贵州大学学报（自然科学版）》2009年第3期18-20,24,共4页Journal of Guizhou University:Natural Sciences

基　　金：国家自然科学基金项目(20662004)

摘　　要：以人前列腺癌PC3细胞作为研究对象,探讨了CCK-8法和MTT法的最佳实验条件。CCK-8的最佳检测波长为450 nm,最佳检测时间为加入CCK-8试剂后4 h,适宜细胞数量范围为8×102-1×105个。在检测抗肿瘤药物阿霉素(ADM)对PC3细胞的增殖影响实验中,发现CCK-8法测得数据的线性相关性优于MTT法,并且数据偏差较MTT法小。实验结果表明CCK-8法较MTT法检测的灵敏度高,准确性好,是一种优于MTT法的检测细胞增殖活性的方法。The optimal experiment conditions of CCK-8 and MTT in PC3 cell proliferation assays were investigated. For CCK,8 assay, the best condition is ： 450 nm for detecting wave length, detected at 4 h after treatment, the number of PC3 cells is around 8 × 10^2 - 1 × 10^5. The antiproliferation action of ADM on PC3 were tested by CCK-8 and MTT. It was found that the result of CCK-8 method has better linear eorrelation fact, smaller deviation, higher sensitivity and accuracy. The conclusion is that CCK-8 method is superior to MTr method in cell proliferation assays.

关 键 词：CCK-8 MTT 前列腺癌 细胞增殖 

分 类 号：Q2-33[生物学—细胞生物学]