胶束电动毛细管色谱法测定速效伤风胶囊组分  

Separation and determination of three ingredients in capsule of Suxiaoshangfeng by micellar electrokinetic capillary chromatography

作　　者：柴逸峰[1,2] 殷学平[1,2] 纪松岗 吴玉田[1,2] 梁东升[1,2] 许自明 李晓[1,2] 

机构地区：[1]第二军医大学药学院药物分析测试中心 [2]海军第401医院药剂科

出　　处：《第二军医大学学报》1998年第3期244-246,共3页Academic Journal of Second Military Medical University

摘　　要：目的：应用胶束电动毛细管色谱法分离测定速效伤风胶囊中的对乙酰氨基酚、氯苯那敏和咖啡因３种组分的含量。方法：对缓冲液ｐＨ和去氧胆酸钠（ＳＤＣ）浓度优化选择，样品经超声振荡、水提取后进样测定。结果：缓冲液为含５０ｍｍｏｌ／ＬＳＤＣ的２０ｍｍｏｌ／ＬＨ３ＢＯ３－ＮａＯＨ（ｐＨ１０），在９ｍｉｎ内将速效伤风胶囊中的上述３种组分完全分离测定，且其迁移时间具有良好的重现性。结论：本法准确、快速和简便，具有良好的线性关系和重现性。Objective: To set up a micellar electrokinetic capillary chromatographic method for the separation and determination of contents of paracetamol, chlorpheniramine and caffein in capsule of Suxiaoshangfeng. Methods: The effects of pH and sodium deoxycholate (SDC) concentrations of running buffer on separation and determination of three ingredients were studied. The samples were extracted with water by ultrasonic agitation and injected directly for determination. Results: The three ingredients mentioned above in capsule of Suxiaoshangfeng were separated completely within 9 min with good reproducibility of migration time in the buffer solution of 20 mmol/L H3BO3NaOH(pH 10) containing 50 mmol/L SDC. Conclusion: The established method is accurate, rapid, simple and has good linearity and reproducibility, providing a new analytical means for the quality control of capsule of Suxiaoshangfeng. 

关 键 词：毛细管色谱法 速效伤风胶囊 MECC 

分 类 号：R971.1[医药卫生—药品]