广东水稻白叶枯病菌遗传多样性和小种分化研究  

Genetic diversity and variability of Xanthomonas oryzae pv. oryzae in Guang-dong

作　　者：曾列先[1] 陈深[1] 张慧[1,2] 潘汝谦[2] 杨健源[1] 伍圣远[1] 翟培茹[1] 朱小源[1] 

机构地区：[1]广东省农业科学院植物保护研究所,广州510640 [2]华南农业大学资源环境学院,广州510642

出　　处：《植物病理学报》2009年第3期231-237,共7页Acta Phytopathologica Sinica

基　　金：广东省自然科学基金重点项目(05103389);农业部农业行业科研专项经费项目(nyhyzx07-056);广东省农业攻关项目(2007A020100004-1);国家科技支撑计划(2006BAD08A04-05);广东省农业科学院重点基础研究项目(07-基础-05)

摘　　要：通过IS-PCR和rep-PCR指纹技术,分析了广东水稻白叶枯病菌(Xanthomonas oryzae pv.oryzae)群体遗传多样性。用2对特异性引物J3和ERIC对114个菌株基因组DNA进行了PCR扩增,分别呈现89和40种谱型,以彼此间的带位相似率达70%为界,J3扩增的谱型被分为11簇,ERIC的谱型被分为8簇。J3的簇1包含89个菌株,占总数的78.07%,ERIC的簇1包含52个菌株,占总数的45.61%,均为优势簇群。群体遗传多样性值J3为0.8919,ERIC为0.8278。上述结果表明,广东水稻白叶枯病菌的遗传多样性较高。全部参试菌株接种于含有不同抗性基因近等基因系及高感品种金刚30共6个鉴别品种,被划分为6个小种(X-gd1,X-gd2,X-gd3,X-gd4,X-gd5和X-gd6),X-gd4出现频率最高,为广东省的优势小种。Genetic diversity and variability of Xanthomonas oryzae pv. oryzae （Xoo） strains collected from Guangdong were analyzed by using IS-PCR and rep-PCR fingerprinting techniques. Genomic DNA from 114 strains was amplified with two specific primers J3 and ERIC, 89 and 40 haplotypes were revealed respectively. Dendrograms were generated from the data by using UPGMA analysis. The strains tested were similar each other at a level of 70%, eleven and eight clusters were gruoped with J3 and ERIC respectively. The predominant groups were both of the cluster 1 for each primer J3 and ERIC involving 89 （78. 07% ） and 52 （46.61%） strains, respectively. The genetic diversifies of the population of tested strains were 0. 891 9 （for J3） and 0. 827 8 （for ERIC）, respectively. The results indicated that the genetic diversity of Xoo in Guangdong was various. 114 strains were inoculated with six differential cultivars including near-isogenic rice lines carrying single resistance gene, IRBB14 （ Xa14 ）, IRBB13 （ xa13 ）, IRBB4 （ Xa4 ）, IRBB203 （ Xa3 ）, IRBB5 （xa5）, and a highly susceptible cultivar Jingang 30. Six races, X-gd1, X-gd2, X-gd3, X-gd4, X- gd5 and X-gd6 were identified. Among them, X-gd4 was predominant in Guangdong province.

关 键 词：水稻白叶枯病菌 遗传多样性 小种 IS-PCR REP-PCR 

分 类 号：S435.111.47[农业科学—农业昆虫与害虫防治]