马铃薯腐烂茎线虫28S rDNA-D2/D3区序列分析  

Molecular cloning and sequences analysis of 28S rDNA-D2/D3 regions of Ditylenchus destructor on sweet potato in China

作　　者：于海英[1,2] 彭德良[1] 胡先奇[2] 黄文坤[1] 

机构地区：[1]中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室,北京100193 [2]云南农业大学农业生物多样性与病虫害控制教育部重点实验室,昆明650201

出　　处：《植物病理学报》2009年第3期254-261,共8页Acta Phytopathologica Sinica

基　　金：“973”项目(2009CB119200);国家自然科学基金资助项目(30871627);国家“十一五”科技支撑计划(2006BAD08A14)

摘　　要：我国危害甘薯的马铃薯腐烂茎线虫rDNA-ITS基因片段长度存在A型(900bp)和B型(1100bp)2个类型,A型腐烂茎线虫群体的ITS片段比B型群体少200bp。为了进一步研究这2种类型群体的发育关系,本文用线虫通用引物D2A和D3B对来自国内的21个马铃薯腐烂茎线虫群体和1个韩国的马铃薯腐烂茎线虫28SrDNA-D2/D3区进行了PCR扩增,均产生大小一致的片段,长度约为780bp,克隆、测序后用DNAMAN5.2软件和MEGA4软件进行分析比对,结果表明我国21个马铃薯腐烂茎线虫群体28SrDNA-D2/D3区只有20余个碱基的差异,相似率达97.2%。基于UPGMA构建的系统发育树很好地区分了马铃薯腐烂茎线虫A型和B型群体,展现出了群体的来源及发育关系。Two types of rDNA-ITS （Type A, 900 bp and Type B, 1100 bp） were demonstrated to exist in Ditylenchus destructor populations on sweet potato in China. The D2/D3 regions of 28S rDNA from 22 populations of D. destructor were amplified with universal primer pairs D2A and D3B. All 22 populations yielded one single fragment about 780 bp. Sequence analysis and alignment were conducted by using the software MEGA4 and DNAMANS. 2 and all 22 sequences were submitted at the GenBank. The results showed that sequence differences of D2/D3 region were only in a few sites, and that the similarity was 97.2 %. Two types could be distinguished in the phylogenetic tree by using UPGMA method. The evolutionary relationship between two types and the origin of Ditylenchus species were discussed.

关 键 词：马铃薯腐烂茎线虫 D2/D3区片段 序列分析 

分 类 号：S435.31[农业科学—农业昆虫与害虫防治]