土壤中棉花黄萎病菌快速检测技术研究  

Studies on the detection of defoliating and nondefoliating pathotypes of Verticillium dahliae in soils

作　　者：高峰[1] 刘培源[1] 马江峰[1] 李晖[1] 李国英[1] 

机构地区：[1]石河子大学新疆兵团绿洲生态农业重点实验室,石河子832003

出　　处：《植物病理学报》2009年第3期310-313,共4页Acta Phytopathologica Sinica

基　　金：国家优质棉基地建设项目(20064)

摘　　要：Verticillium wilt,caused by Verticillium dahliae,is the most important disease of cotton.In this work,the previously developed defoliating(D) and nondefoliating(ND) V.dahliae-specific primers were adopted for detection of pathotypes of V.dahliae in soil by using nested PCR.The results showed that the detection assay was efficient when used in infested soil and was an useful technique for rapid and accurate assessment of soil contamination by V.dahliae.Verticillium wilt, caused by Verticillium dahliae, is the most important disease of cotton. In this work, the previously developed defoliating （D） and nondefoliating （ND） V. dahliae-specific primers were adopted for detection of pathotypes of V. dahliae in soil by using nested PCR. The results showed that the detection assay was efficient when used in infested soil and was an useful technique for rapid and accurate assessment of soil contamination by V. dahliae.

关 键 词：棉花黄萎病菌 快速检测技术 土壤 落叶型菌系 致病性分化 鉴定技术 生物学测定 PCR技术 

分 类 号：S435.621.2[农业科学—农业昆虫与害虫防治] TS207.4[农业科学—植物保护]