香蕉果实炭疽病病程相关基因cDNA片段克隆  

Cloning of banana fruit anthracnose pathogenesis-related cDNA

作　　者：陈弟[1] 谭志琼[1] 殷晓敏[1] 徐碧玉[2] 金志强[2] 张荣意[1] 

机构地区：[1]海南大学环境与植物保护学院,儋州571737 [2]中国热带农业科学院热带生物技术研究所,海口571101

出　　处：《植物病理学报》2009年第3期325-327,共3页Acta Phytopathologica Sinica

基　　金：海南大学博士启动基金(Rndy0602)

摘　　要：A suppression subtractive hybridization(SSH) cDNA library was constructed from 5 d lesion of banana fruit inoculated with Colletotrichum musae F1.70 cDNA clones selected randomly were sequenced and analysed.The result showed that 29 cDNA clones were related to disease resistance.They were chitinase,se-rine/threonine phosphate,Leucine-rich repeat(LRR),isoflavone reductase and so on.A suppression subtractive hybridization （SSH） cDNA library was constructed from 5 d lesion of banana fruit inoculated with Colletotrichum musae F1. 70 cDNA clones selected randomly were sequenced and analysed. The result showed that 29 cDNA clones were related to disease resistance. They were chitinase, serine/threonine phosphate,Leucine-rich repeat（LRR） ,isoflavone reductase and so on.

关 键 词：香蕉炭疽病 病程相关基因 香蕉果实 CDNA片段 克隆 防治技术 香蕉生产 抗病品种 

分 类 号：S436.68[农业科学—农业昆虫与害虫防治] Q943[农业科学—植物保护]