芝芪菌质多糖的分离、纯化及体外抗肿瘤活性初步研究  

Preparation of Zhiqi Fungal Substance Polysaccharides and Research on their Primary Antitumor Activities

作　　者：张雅雯[1] 唐宁[1] 饶玉鹏[1] 张敦林[1] 

机构地区：[1]南京晓庄学院生命科学系,江苏南京211171

出　　处：《安徽农学通报》2009年第13期40-41,共2页Anhui Agricultural Science Bulletin

基　　金：南京晓庄学院院级科研项目青年专项(2007NXY28)

摘　　要：采用水提、醇沉、离子交换层析等方法分离纯化芝芪菌质多糖,并对各纯化多糖成分进行体外肿瘤细胞增殖的抑制活性测定。用DEAE-32纤维素柱层析分离得到4种纯化的芝芪菌质多糖GAP-1、GAP-2、GAP-3、GAP-4。4种纯化的芝芪菌质多糖均有一定程度的体外抗肿瘤活性,其中适宜浓度的GAP-3的体外肿瘤细胞增殖的抑制活性最好。芝芪菌质纯化多糖对GAP-3K562、SMMC-7721和PC-3细胞增殖抑制作用明显,有一定的抗肿瘤药开发前景。To separate and purify the Zhiqi fungal substance polysaccharides by water extraction, methanol deposition and ion - exchange chromatography, then the effects of the purified polysaccharides on the mice cells＇ vitro antitumor activities was preliminary determined. Four purified polysaccbarides GAP - 1, GAP -2, GAP -3, GAP -4 were obtained. The four purified polysaccharides could promote vitro antitumor activities at certain extent, and GAP -3 showed the best activities. GAP- 3 has relatively high Antitumor activities, and it could be a promising antitumor drug.

关 键 词：芝芪菌质 纯化多糖 抗肿瘤活性 

分 类 号：R392[医药卫生—免疫学]