龙吐珠组培快繁技术初探  

Discussion on the Tissue Culture and Rapid Propagation Techniques of Clerodendrum thomsonae Balf.

作　　者：李鹤春[1] 李成雁[1] 庄金慧[1] 李凤兰[1] 胡国富[1] 

机构地区：[1]东北农业大学生命科学学院,黑龙江哈尔滨150030

出　　处：《安徽农业科学》2009年第21期9863-9866,共4页Journal of Anhui Agricultural Sciences

摘　　要：[目的]探究龙吐珠最佳组织培养方案。[方法]以马鞭草科龙吐珠叶片、茎段、腋芽为外植体,进行组培快繁技术研究。[结果]最适宜外植体为茎段;最适宜消毒处理是消毒剂为0.1%升汞溶液,消毒时间为6 min。产生侧芽的最佳培养基为1/2 MS+6-BA1.00mg/L+NAA0.05 mg/L+IBA0.50 mg/L,最适愈伤组织诱导及继代培养基为1/2 MS+6-BA2.00 mg/L+NAA0.10 mg/L,平均诱导率为86.2%;最适分化培养基是1/2 MS+6-BA1.00 mg/L+NAA0.10 mg/L+KT0.20 mg/L,平均分化率为76.7%;不定芽的最适生根培养基是1/2 MS+NAA0.01 mg/L,其生根率为100%。[结论]该方案可为龙吐珠规模化生产提供技术支持。[Objective] This research aimed to find out the best tissue culture program of Clerodendrum thomsonae Balf. [ Method] With the leaves, stem segments, axillary buds of C. thomsonae as explants, the tissue culture and rapid propagation techniques were studied. [ Result] The optimum disinfection method was 0.1% solution of mercuric chloride as the disinfectant, and the disinfection course lasted 6 minutes. The best nutrient medium to produce lateral bud was 1/2 MS ＋ 1.00 mg/L 6-BA ＋0.05 mg/L NAA ＋0.50 mg/L IBA. The optimum cultivating medium of inducement callus and successive transfer culture was to improve 1/2 MS ＋ 2.00 mg/L 6-BA ＋ 0.10 mg/L NAA, and its average rate of inducement was 86.2%. The optimum culture medimn of differentiation for calli was 1/2 MS ＋ 1.00 mg/L 6-BA ＋0.10 mg/L NAA ＋ 0.20 mg/L KT, and its average rate of differentiation was 76.7%. The optimum culture medium of rooting was 1/2 MS ＋0.01 mg/L NAA, and its average rate of rooting was 100%. [ Conclusion] This program can provide technical support for the scale production of Clerodendrum thomsonae Balf.

关 键 词：龙吐珠 组织培养 茎段 

分 类 号：S603.6[农业科学—园艺学]